For the input data seu, up to which step should it be prepared:
seu<- scobj %>%
NormalizeData() %>%
FindVariableFeatures() %>%
ScaleData() %>%
RunPCA() %>%
RunUMAP(dims = 1:10) %>%
FindNeighbors() %>%
FindClusters(resolution=0.5)
need to remove batch effects from data originating from different samples?
For the input data seu, up to which step should it be prepared: seu<- scobj %>% NormalizeData() %>% FindVariableFeatures() %>% ScaleData() %>% RunPCA() %>% RunUMAP(dims = 1:10) %>% FindNeighbors() %>% FindClusters(resolution=0.5)
need to remove batch effects from data originating from different samples?