library("DoubletFinder")#注意一定要先运行
source("./scRNA_scripts/doubletFinder_v3.R")
source("scRNA_scripts/paramSweep_v3.R")
View(sce.all.int)
View(sce.all.int@meta.data)
sce.all.int<- scutilsR::MarkDoublets(sce.all.int, split.by = "orig.ident")
| | 0 % ~calculating 1. Preprocessing ...
Normalize ...
Warning: Cannot find a parent environment called Seurat
Find variable genes ...
Scale data ...
Warning: Different features in new layer data than already exists for scale.data
Run PCA ...
Find neighbors ...
Error: std::bad_alloc
老师好,我在跑去双细胞过程时遇到了以下报错,这应该是发生在你fix上一个有关的bug后
devtools::install_github('chris-mcginnis-ucsf/DoubletFinder')