Methylation mismatches look like T and C by our initial design, idea was that unmethylated cytosine coloured like ordinary cytosine and methylated cytosine looks like changed cytosine. Also, it is consistent with IGV and often people show methylated CpG using red/green/black colors and not methylated using blue/white colours. Maybe the intuition of colors selection is a bit misleading, because red is color of T and T are mismatches where cytosine wasn't protected by methylation, so maybe other color scheme will be easier to memorise.
On the other hand, we want to visualize mismatches that cannot be explained by bisulfite treatment., e.g. to highlight SNP and combination for SNP+methylaiton.
At the moment, SNP looks like methylation:
Let's fix it. Suggestions:
change the shape of the mismatch bar, e.g. make methylation-related bars shorter with equal gaps above and below the bar
bars will be still visible in coverage in a same way
do not show non-methylated mismatches in coverage
bars in reads will be still look same + SNP will be less noticeable
Use colours that different from A/T/G/C/N colors , e.g.:
this == METHYLATED_C -> Color(255,80,180)
this == UNMETHYLATED_C -> Color(0 ,100,250)
Methylation mismatches look like T and C by our initial design, idea was that unmethylated cytosine coloured like ordinary cytosine and methylated cytosine looks like changed cytosine. Also, it is consistent with IGV and often people show methylated CpG using red/green/black colors and not methylated using blue/white colours. Maybe the intuition of colors selection is a bit misleading, because red is color of
T
andT
are mismatches where cytosine wasn't protected by methylation, so maybe other color scheme will be easier to memorise.On the other hand, we want to visualize mismatches that cannot be explained by bisulfite treatment., e.g. to highlight SNP and combination for SNP+methylaiton.
At the moment, SNP looks like methylation:
Let's fix it. Suggestions:
and with reads coloring: