runPseudotime Error: cannot locate a vector of size 11.9 Gb

JhuangLab / CytoTree

A Toolkit for Construct Tree-shaped Trajectory for Flow and Mass Cytometry data

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runPseudotime Error: cannot locate a vector of size 11.9 Gb #6

Open Hgracia opened 2 years ago

Hgracia commented 2 years ago

Hello,

I have this cyt object: CYT Information: Input cell number: 62000 cells Enroll marker number: 42 markers Cells after downsampling: 62000 cells

It have a memory size of 185.8 MB

The error raises when i follow the # Standard workflow of CytoTree cyt <- runPseudotime(cyt) 2021-12-14 18:04:07 Pseudotime exists in meta.data, it will be replaced. Error: cannot locate a vector of size 11.9 Gb

Ussing CytoTree_1.2.0 and 32GB of RAM memory. Seems that something is leaking

Taimie48 commented 2 years ago

I have the same problem. Did you find any solutions yet? Best greetings

ytdai commented 2 years ago

Sorry for the late reply. I think you can try to downsample the input cell number. Thank you.