Hi all, I am trying scTE on some scRNA-seq data of mine (hg38). I have BAM files generated with STARSolo and following the instructions I'm quantifying TE like this:
All samples are being processed but in some log files I'm finding this message:
[…]
INFO : Loading the genome annotation index... 2021-11-18 11:13:28
INFO : Loaded '/beegfs/scratch/ric.cosr/cittaro.davide/Ref/scTE/hg38/hg38.exclusive.idx' binary file with 4779764 items
INFO : Finished loading the genome annotation index... 2021-11-18 11:14:06
INFO : Processing BAM/SAM files ...2021-11-18 11:14:06
INFO : Input SAM/BAM file appears to be valid
sed: couldn't write 50 items to stdout: Broken pipe
sed: couldn't write 53 items to stdout: Broken pipe
sed: couldn't write 58 items to stdout: Broken pipe
awk: cmd. line:1: (FILENAME=- FNR=131567431) fatal: print to "standard output" failed (Broken pipe)
The forked process
samtools view -@ 8 HCT116_FOLFIRI_LTAligned.sortedByCoord.out.bam | awk '{OFS="?"}{for(i=12;i<=NF;i++)if($i~/CR:Z:/)n=i}{for(i=12;i<=NF;i++)if($i~/UR:Z:/)m=i}{print $3,$4,$4+100,$n,$m}' | sed -r 's/CR:Z://g' | sed -r 's/UR:Z://g'| sed -r 's/^chr//g' | awk '!x[$4$5]++' | gzip -c > HCT116_FOLFIRI_LT_TE_scTEtmp/o1/HCT116_FOLFIRI_LT_TE.bed.gz
is still running (apparently) but, compared to other processes launched in the same moment, it seems I'm stuck in generating the content of o1 folder. Any hint?
Hi all, I am trying scTE on some scRNA-seq data of mine (hg38). I have BAM files generated with STARSolo and following the instructions I'm quantifying TE like this:
All samples are being processed but in some log files I'm finding this message:
The forked process
is still running (apparently) but, compared to other processes launched in the same moment, it seems I'm stuck in generating the content of
o1
folder. Any hint?