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JimboMahoney / CyTOF-Template-Creator

Shiny interface to create CyTOF .tem files
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Would be sweet to improve spillover channels ("Blanks") to include isotopes. #5

Closed JimboMahoney closed 4 years ago

JimboMahoney commented 4 years ago

There are three sources of "spillover" in mass cytometry:

1) Abundance sensitivity (M +/- 1) due to very "loud" markers, or overstaining. 2) M+16 from oxidation. 3) Isotopic impurities - all metals for conjugation are less than 100% pure, therefore other isotopes will always be present. e.g. Pd is present in 6 isotopes - 102, 104, 105, 106, 108 and 110. When using a particular isotope, the others will be present in small amounts. A "loud" enough marker will cause "spill" into other isotope channels.

It would therefore be awesome to have a list of every isotope (I already have this) and program a method to add all isotopes that are present in the panel....

The "Blanks" currently only accounts for abundance sensitivity.

JimboMahoney commented 4 years ago

OK, this is now done. Adding blanks will add both M+/-1 and isotopic impurities.