There are three sources of "spillover" in mass cytometry:
1) Abundance sensitivity (M +/- 1) due to very "loud" markers, or overstaining.
2) M+16 from oxidation.
3) Isotopic impurities - all metals for conjugation are less than 100% pure, therefore other isotopes will always be present. e.g. Pd is present in 6 isotopes - 102, 104, 105, 106, 108 and 110. When using a particular isotope, the others will be present in small amounts. A "loud" enough marker will cause "spill" into other isotope channels.
It would therefore be awesome to have a list of every isotope (I already have this) and program a method to add all isotopes that are present in the panel....
The "Blanks" currently only accounts for abundance sensitivity.
There are three sources of "spillover" in mass cytometry:
1) Abundance sensitivity (M +/- 1) due to very "loud" markers, or overstaining. 2) M+16 from oxidation. 3) Isotopic impurities - all metals for conjugation are less than 100% pure, therefore other isotopes will always be present. e.g. Pd is present in 6 isotopes - 102, 104, 105, 106, 108 and 110. When using a particular isotope, the others will be present in small amounts. A "loud" enough marker will cause "spill" into other isotope channels.
It would therefore be awesome to have a list of every isotope (I already have this) and program a method to add all isotopes that are present in the panel....
The "Blanks" currently only accounts for abundance sensitivity.