JinmiaoChenLab / cytofkit

cytofkit: an integrated flow/mass cytometry data analysis pipeline
http://jinmiaochenlab.github.io/cytofkit/
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[suggestion] Include cluster ID (integer) in analyzedFCS output #8

Closed esimonds closed 7 years ago

esimonds commented 7 years ago

When doing PhenoGraph clustering in Cytofkit, the FCS file output to the "analyzedFCS" folder contains some useful new parameters. For example:

... $P44 tsne_1 74 0.00000000 75 $P45 tsne_2 80 0.00000000 81 $P46 Rphenograph_cor_1 3178 -0.08243132 3178 $P47 Rphenograph_cor_2 3178 -0.08243132 3178 363 keywords are stored in the 'description' slot

...however, it does not contain the cluster IDs. It would be very handy if these were added as the final column of the FCS file, as integers corresponding to the cluster IDs in the interactive Cytofkit display.

jinmiaochen commented 7 years ago

Dear Erin,

Thanks a lot for your feedback. We will add the cluster ID as integers to FCS in our next version.

Best, Jinmiao

From: Erin Simonds [mailto:notifications@github.com] Sent: Wednesday, July 26, 2017 8:09 AM To: JinmiaoChenLab/cytofkit cytofkit@noreply.github.com Cc: Subscribed subscribed@noreply.github.com Subject: [JinmiaoChenLab/cytofkit] [suggestion] Include cluster ID (integer) in analyzedFCS output (#8)

When doing PhenoGraph clustering in Cytofkit, the FCS file output to the "analyzedFCS" folder contains some useful new parameters. For example:

... $P44 tsne_1 74 0.00000000 75 $P45 tsne_2 80 0.00000000 81 $P46 Rphenograph_cor_1 3178 -0.08243132 3178 $P47 Rphenograph_cor_2 3178 -0.08243132 3178 363 keywords are stored in the 'description' slot

...however, it does not contain the cluster IDs. It would be very handy if these were added as the final column of the FCS file, as integers corresponding to the cluster IDs in the interactive Cytofkit display.

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MattMyint commented 7 years ago

Hi Erin!

Its taken a while, but we've incorporated your suggestion into cytofkit! You can try it on the devel branch (i.e. this github repo)

Let me know if there's any issues!

Regards Matthew

esimonds commented 7 years ago

Thanks, I'm excited to try it!