Open caozilongsuper opened 1 year ago
Hmm, what is the first line or so of finngen_adeno_tomtag.txt? (the column headers) Just trying to see why it would be having trouble identifying columns.
On Mon, Oct 16, 2023 at 9:58 AM caozilongsupper @.***> wrote:
I have prepared the format files such as chr,bpos,a1,a2,snpid,beta,se,pval,freq,N,z.When I run the MTAG, I am faced with an error. Here is the log: Calling ./mtag.py --stream-stdout --n-min 0.0 --sumstats finngen_adeno_tomtag.txt,finngen_squam_tomtag.txt,finngen_uteri_tomtag.txt --out /share/CZL/result/cancer/Cervix/finngen_mtag.1NS
Beginning MTAG analysis... MTAG will use the Z column for analyses. Read in Trait 1 summary statistics (20155970 SNPs) from finngen_adeno_tomtag.txt ...
<><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> Munging Trait 1 <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><
<><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><>
ERROR converting summary statistics:
Traceback (most recent call last): File "/share/CZL/MTAG/mtag-master/mtag_munge.py", line 802, in munge_sumstats raise ValueError('Could not find {C} column.'.format(C=c)) ValueError: Could not find SNP column.
Conversion finished at Mon Oct 16 21:29:12 2023 Total time elapsed: 0.01s Could not find SNP column. Traceback (most recent call last): File "/share/CZL/MTAG/mtag-master/mtag.py", line 1577, in mtag(args) File "/share/CZL/MTAG/mtag-master/mtag.py", line 1343, in mtag DATA_U, DATA, args = load_and_merge_data(args) File "/share/CZL/MTAG/mtag-master/mtag.py", line 273, in load_and_merge_data GWAS_d[p], sumstats_format[p] = _perform_munge(args, GWAS_d[p], gwas_dat_gen, p) File "/share/CZL/MTAG/mtag-master/mtag.py", line 166, in _perform_munge munged_results = munge_sumstats.munge_sumstats(argnames, write_out=False, new_log=False) File "/share/CZL/MTAG/mtag-master/mtag_munge.py", line 802, in munge_sumstats raise ValueError('Could not find {C} column.'.format(C=c)) ValueError: Could not find SNP column. Analysis terminated from error at Mon Oct 16 21:29:12 2023 Total time elapsed: 43.09s It is much appreciated if you could help me solve such problems!
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Thanks for replying,and here is the information. [TCGA@cu03 ~]$ head /share/CZL/pancancer-GWAS/cervix_carcinoma/finngen/finngen_adeno_tomtag.txt chr,bpos,a1,a2,snpid,beta,se,pval,freq,N,z 1,13668,A,G,rs2691328,0.389358,1.61109,0.809033,0.0058238,167301,0.241673649516787 1,14773,T,C,rs878915777,-1.15586,1.008,0.251513,0.0134509,167301,-1.14668650793651 1,15585,A,G,rs533630043,-1.96825,3.15742,0.53304,0.00109271,167301,-0.623372880389685 1,16549,C,T,rs1262014613,-0.318079,4.5183,0.943877,0.000589333,167301,-0.0703979372772946 1,16567,C,G,rs1194064194,1.34032,1.62093,0.408303,0.00422919,167301,0.826883332407939 1,16792,A,G,rs201330479,1.98142,2.53122,0.433749,0.0012803,167301,0.782792487417135 1,16850,C,T,rs1298041101,-0.768738,5.56921,0.890214,0.000259096,167301,-0.13803358106446 1,16869,T,C,rs1201088959,-2.8915,13.1371,0.825792,8.19235e-05,167301,-0.2201018489621 1,17017,G,C,,8.97029,9.22849,0.33104,9.60228e-05,167301,0.972021424956846
Ah, I think you may want to try a whitespace-delimited file (rather than commas) as per this portion of the wiki https://github.com/JonJala/mtag/wiki/Tutorial-1:-The-Basics#sample-gwas-results-and-data-format. I'm guessing that's why it's having trouble identifying the columns.
On Tue, Oct 17, 2023 at 10:16 PM caozilongsupper @.***> wrote:
Thanks for replying,and here is the information. @.*** ~]$ head /share/CZL/pancancer-GWAS/cervix_carcinoma/finngen/finngen_adeno_tomtag.txt chr,bpos,a1,a2,snpid,beta,se,pval,freq,N,z
1,13668,A,G,rs2691328,0.389358,1.61109,0.809033,0.0058238,167301,0.241673649516787
1,14773,T,C,rs878915777,-1.15586,1.008,0.251513,0.0134509,167301,-1.14668650793651
1,15585,A,G,rs533630043,-1.96825,3.15742,0.53304,0.00109271,167301,-0.623372880389685
1,16549,C,T,rs1262014613,-0.318079,4.5183,0.943877,0.000589333,167301,-0.0703979372772946
1,16567,C,G,rs1194064194,1.34032,1.62093,0.408303,0.00422919,167301,0.826883332407939
1,16792,A,G,rs201330479,1.98142,2.53122,0.433749,0.0012803,167301,0.782792487417135
1,16850,C,T,rs1298041101,-0.768738,5.56921,0.890214,0.000259096,167301,-0.13803358106446
1,16869,T,C,rs1201088959,-2.8915,13.1371,0.825792,8.19235e-05,167301,-0.2201018489621 1,17017,G,C,,8.97029,9.22849,0.33104,9.60228e-05,167301,0.972021424956846
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Thanks for offering help and first error has been solved. But here comes another problem. Read 20156086 SNPs from --sumstats file. Removed 0 SNPs with missing values. Removed 0 SNPs with INFO <= None. Removed 0 SNPs with MAF <= 0.01. Removed 0 SNPs with SE <0 or NaN values. Removed 1362973 SNPs with out-of-bounds p-values. Removed 1400697 variants that were not SNPs. Note: strand ambiguous SNPs were not dropped. 17392416 SNPs remain. Removed 58485 SNPs with duplicated rs numbers (17333931 SNPs remain). Removed 0 SNPs with N < 0.0 (17333931 SNPs remain).
ERROR converting summary statistics:
Traceback (most recent call last): File "/home/CZL/software/MTAG/mtag-master/mtag_munge.py", line 882, in munge_sumstats check_median(dat.SIGNED_SUMSTAT, signed_sumstat_null, args.median_z_cutoff, sign_cname)) File "/home/CZL/software/MTAG/mtag-master/mtag_munge.py", line 525, in check_median raise ValueError(msg.format(F=name, M=expected_median, V=round(m, 2))) ValueError: WARNING: median value of SIGNED_SUMSTAT is -0.22 (should be close to 0.0). This column may be mislabeled.
You could try making use of the median_z_cutoff flag to relax the threshold on that check:
https://github.com/JonJala/mtag/blob/master/mtag.py#L1540C4-L1540C4
On Wed, Oct 25, 2023 at 9:11 AM caozilongsupper @.***> wrote:
Thanks for offering help and first error has been solved. But here comes another problem. Read 20156086 SNPs from --sumstats file. Removed 0 SNPs with missing values. Removed 0 SNPs with INFO <= None. Removed 0 SNPs with MAF <= 0.01. Removed 0 SNPs with SE <0 or NaN values. Removed 1362973 SNPs with out-of-bounds p-values. Removed 1400697 variants that were not SNPs. Note: strand ambiguous SNPs were not dropped. 17392416 SNPs remain. Removed 58485 SNPs with duplicated rs numbers (17333931 SNPs remain). Removed 0 SNPs with N < 0.0 (17333931 SNPs remain).
ERROR converting summary statistics:
Traceback (most recent call last): File "/home/CZL/software/MTAG/mtag-master/mtag_munge.py", line 882, in munge_sumstats check_median(dat.SIGNED_SUMSTAT, signed_sumstat_null, args.median_z_cutoff, sign_cname)) File "/home/CZL/software/MTAG/mtag-master/mtag_munge.py", line 525, in check_median raise ValueError(msg.format(F=name, M=expected_median, V=round(m, 2))) ValueError: WARNING: median value of SIGNED_SUMSTAT is -0.22 (should be close to 0.0). This column may be mislabeled.
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Add it in the code just like this? python /home/CZL/software/MTAG/mtag-master/mtag.py \ --sumstats finngen_adeno_tomtag1.txt,finngen_squam_tomtag1.txt,finngen_uteri_tomtag1.txt \ --median_z_cutoff --out /share/CZL/result/cancer/Cervix/finngen_mtag.1NS \ --n_min 0.0 \ --stream_stdout Thank you in advance!
Hi JonJala,
I have fixed SIGNED_SUMSTAT problem using --median_z_cutoff, but here comes another one.
Total time elapsed: 3.0m:36.14s
'snpid'
Traceback (most recent call last):
File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 1577, in
Do you mind including the whole log? It might help to see what came before.
On Thu, Oct 26, 2023 at 5:14 AM caozilongsupper @.***> wrote:
Hi JonJala, I have fixed SIGNED_SUMSTAT problem using --median_z_cutoff, but here comes another one. Total time elapsed: 3.0m:36.14s 'snpid' Traceback (most recent call last): File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 1577, in mtag(args) File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 1343, in mtag DATA_U, DATA, args = load_and_merge_data(args) File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 273, in load_and_merge_data GWAS_d[p], sumstats_format[p] = _perform_munge(args, GWAS_d[p], gwas_dat_gen, p) File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 167, in _perform_munge GWAS_df = GWAS_df.merge(munged_results, how='inner',left_on =args.snp_name,right_on='SNP',suffixes=('','_ss')) File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/frame.py", line 6868, in merge copy=copy, indicator=indicator, validate=validate) File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/reshape/merge.py", line 47, in merge validate=validate) File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/reshape/merge.py", line 529, in init self.join_names) = self._get_merge_keys() File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/reshape/merge.py", line 846, in _get_merge_keys left_keys.append(left._get_label_or_level_values(lk)) File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/generic.py", line 1706, in _get_label_or_level_values raise KeyError(key) KeyError: 'snpid' Analysis terminated from error at Thu Oct 26 17:05:34 2023 Total time elapsed: 4.0m:40.98s Sincerely thanks in advance!
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This is the whole log: <> MTAG: Multi-trait Analysis of GWAS <> Version: 1.0.8 <> (C) 2017 Omeed Maghzian, Raymond Walters, and Patrick Turley <> Harvard University Department of Economics / Broad Institute of MIT and Harvard <> GNU General Public License v3 <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> <> Note: It is recommended to run your own QC on the input before using this program. <> Software-related correspondence: jjala.ssgac@gmail.com <> All other correspondence: paturley@broadinstitute.org <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><>
Calling ./mtag.py \ --stream-stdout \ --n-min 0.0 \ --median-z-cutoff 1.0 \ --sumstats finngen_adeno_tomtag1.txt,finngen_squam_tomtag1.txt,finngen_uteri_tomtag1.txt \ --out /share/CZL/result/cancer/Cervix/finngen_mtag.1NS
Beginning MTAG analysis... WARNING: Using non-default median Z score cutoff for QC. MTAG will use the Z column for analyses. Read in Trait 1 summary statistics (20156086 SNPs) from finngen_adeno_tomtag1.txt ... <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> Munging Trait 1 <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><>< <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> Interpreting column names as follows: N: Sample size a1: a1, interpreted as ref allele for signed sumstat. pval: p-Value a2: a2, interpreted as non-ref allele for signed sumstat. SNP: Variant ID (e.g., rs number) z: Directional summary statistic as specified by --signed-sumstats. se: Standard errors of BETA coefficients
Reading sumstats from provided DataFrame into memory 10000000 SNPs at a time. WARNING: 327274 SNPs had P outside of (0,1]. The P column may be mislabeled. WARNING: 879613 SNPs had P outside of (0,1]. The P column may be mislabeled. WARNING: 156086 SNPs had P outside of (0,1]. The P column may be mislabeled. Read 20156086 SNPs from --sumstats file. Removed 0 SNPs with missing values. Removed 0 SNPs with INFO <= None. Removed 0 SNPs with MAF <= 0.01. Removed 0 SNPs with SE <0 or NaN values. Removed 1362973 SNPs with out-of-bounds p-values. Removed 1400697 variants that were not SNPs. Note: strand ambiguous SNPs were not dropped. 17392416 SNPs remain. Removed 58485 SNPs with duplicated rs numbers (17333931 SNPs remain). Removed 0 SNPs with N < 0.0 (17333931 SNPs remain). Median value of SIGNED_SUMSTAT was -0.215595440827, which seems sensible. Dropping snps with null values
Metadata: Mean chi^2 = 0.93 WARNING: mean chi^2 may be too small. Lambda GC = 0.86 Max chi^2 = 29.088 1 Genome-wide significant SNPs (some may have been removed by filtering).
Conversion finished at Sat Oct 28 21:07:43 2023
Total time elapsed: 6.0m:53.26s
'snpid'
Traceback (most recent call last):
File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 1577, in
Hmm, it's unclear what's going on there. Have you switched all your files to be whitespace-delimited? And there is a "snpid" column in each?
On Sat, Oct 28, 2023 at 9:14 AM caozilongsupper @.***> wrote:
This is the whole log: <> MTAG: Multi-trait Analysis of GWAS <> Version: 1.0.8 <> (C) 2017 Omeed Maghzian, Raymond Walters, and Patrick Turley <> Harvard University Department of Economics / Broad Institute of MIT and Harvard <> GNU General Public License v3
<><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> <> Note: It is recommended to run your own QC on the input before using this program. <> Software-related correspondence: @. <> All other correspondence: @.
<><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><>
Calling ./mtag.py --stream-stdout --n-min 0.0 --median-z-cutoff 1.0 --sumstats finngen_adeno_tomtag1.txt,finngen_squam_tomtag1.txt,finngen_uteri_tomtag1.txt
--out /share/CZL/result/cancer/Cervix/finngen_mtag.1NS
Beginning MTAG analysis... WARNING: Using non-default median Z score cutoff for QC. MTAG will use the Z column for analyses. Read in Trait 1 summary statistics (20156086 SNPs) from finngen_adeno_tomtag1.txt ...
<><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> Munging Trait 1 <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><
<><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> Interpreting column names as follows: N: Sample size a1: a1, interpreted as ref allele for signed sumstat. pval: p-Value a2: a2, interpreted as non-ref allele for signed sumstat. SNP: Variant ID (e.g., rs number) z: Directional summary statistic as specified by --signed-sumstats. se: Standard errors of BETA coefficients
Reading sumstats from provided DataFrame into memory 10000000 SNPs at a time. WARNING: 327274 SNPs had P outside of (0,1]. The P column may be mislabeled. WARNING: 879613 SNPs had P outside of (0,1]. The P column may be mislabeled. WARNING: 156086 SNPs had P outside of (0,1]. The P column may be mislabeled. Read 20156086 SNPs from --sumstats file. Removed 0 SNPs with missing values. Removed 0 SNPs with INFO <= None. Removed 0 SNPs with MAF <= 0.01. Removed 0 SNPs with SE <0 or NaN values. Removed 1362973 SNPs with out-of-bounds p-values. Removed 1400697 variants that were not SNPs. Note: strand ambiguous SNPs were not dropped. 17392416 SNPs remain. Removed 58485 SNPs with duplicated rs numbers (17333931 SNPs remain). Removed 0 SNPs with N < 0.0 (17333931 SNPs remain). Median value of SIGNED_SUMSTAT was -0.215595440827, which seems sensible. Dropping snps with null values
Metadata: Mean chi^2 = 0.93 WARNING: mean chi^2 may be too small. Lambda GC = 0.86 Max chi^2 = 29.088 1 Genome-wide significant SNPs (some may have been removed by filtering).
Conversion finished at Sat Oct 28 21:07:43 2023 Total time elapsed: 6.0m:53.26s 'snpid' Traceback (most recent call last): File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 1577, in mtag(args) File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 1343, in mtag DATA_U, DATA, args = load_and_merge_data(args) File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 273, in load_and_merge_data GWAS_d[p], sumstats_format[p] = _perform_munge(args, GWAS_d[p], gwas_dat_gen, p) File "/home/CZL/software/MTAG/mtag-master/mtag.py", line 167, in _perform_munge GWAS_df = GWAS_df.merge(munged_results, how='inner',left_on =args.snp_name,right_on='SNP',suffixes=('','_ss')) File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/frame.py", line 6868, in merge copy=copy, indicator=indicator, validate=validate) File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/reshape/merge.py", line 47, in merge validate=validate) File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/reshape/merge.py", line 529, in init self.join_names) = self._get_merge_keys() File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/reshape/merge.py", line 846, in _get_merge_keys left_keys.append(left._get_label_or_level_values(lk)) File "/home/CZL/.conda/envs/czl/lib/python2.7/site-packages/pandas/core/generic.py", line 1706, in _get_label_or_level_values raise KeyError(key) KeyError: 'snpid' Analysis terminated from error at Sat Oct 28 21:07:44 2023 Total time elapsed: 8.0m:29.31s Thank you for offering me critical help!
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There is another question. I have checked my input file data and all pval column is between (0,1]. But I still meet this log: WARNING: 327274 SNPs had P outside of (0,1]. The P column may be mislabeled. WARNING: 879613 SNPs had P outside of (0,1]. The P column may be mislabeled. WARNING: 156086 SNPs had P outside of (0,1]. The P column may be mislabeled.
Sometimes MTAG gets confused about what columns are what. Do you have many more than the required columns in your summary statistics file?
On Tue, Oct 31, 2023, 3:49 AM caozilongsupper @.***> wrote:
There is another question. I have checked my input file data and all pval column is between (0,1]. But I still meet this log: WARNING: 327274 SNPs had P outside of (0,1]. The P column may be mislabeled. WARNING: 879613 SNPs had P outside of (0,1]. The P column may be mislabeled. WARNING: 156086 SNPs had P outside of (0,1]. The P column may be mislabeled.
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Dear paturley, Many thanks for your quick response to all my questions, it is very helpful! Best, Zilong Cao
I have prepared the format files such as chr,bpos,a1,a2,snpid,beta,se,pval,freq,N,z.When I run the MTAG, I am faced with an error. Here is the log: Calling ./mtag.py \ --stream-stdout \ --n-min 0.0 \ --sumstats finngen_adeno_tomtag.txt,finngen_squam_tomtag.txt,finngen_uteri_tomtag.txt \ --out /share/CZL/result/cancer/Cervix/finngen_mtag.1NS
Beginning MTAG analysis... MTAG will use the Z column for analyses. Read in Trait 1 summary statistics (20155970 SNPs) from finngen_adeno_tomtag.txt ... <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><> Munging Trait 1 <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><>< <><><<>><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><><>
ERROR converting summary statistics:
Traceback (most recent call last): File "/share/CZL/MTAG/mtag-master/mtag_munge.py", line 802, in munge_sumstats raise ValueError('Could not find {C} column.'.format(C=c)) ValueError: Could not find SNP column.
Conversion finished at Mon Oct 16 21:29:12 2023 Total time elapsed: 0.01s Could not find SNP column. Traceback (most recent call last): File "/share/CZL/MTAG/mtag-master/mtag.py", line 1577, in
mtag(args)
File "/share/CZL/MTAG/mtag-master/mtag.py", line 1343, in mtag
DATA_U, DATA, args = load_and_merge_data(args)
File "/share/CZL/MTAG/mtag-master/mtag.py", line 273, in load_and_merge_data
GWAS_d[p], sumstats_format[p] = _perform_munge(args, GWAS_d[p], gwas_dat_gen, p)
File "/share/CZL/MTAG/mtag-master/mtag.py", line 166, in _perform_munge
munged_results = munge_sumstats.munge_sumstats(argnames, write_out=False, new_log=False)
File "/share/CZL/MTAG/mtag-master/mtag_munge.py", line 802, in munge_sumstats
raise ValueError('Could not find {C} column.'.format(C=c))
ValueError: Could not find SNP column.
Analysis terminated from error at Mon Oct 16 21:29:12 2023
Total time elapsed: 43.09s
It is much appreciated if you could help me solve such problems!