I have the UMI count matrix (genes by barcodes) from a mixed species experiments. The same barcode might also have few UMI counts in both human and mouse. Currently, based on few reference articles, I have got the mouse ortholog genes in human and have a data for these common genes. Essentially, two count matrix, where gene names are same and barcodes with UMI counts. How do I use this in DoubletDetection to estimate doublets.
Kindly suggest.
Thanks.
Hi @JonathanShor ,
I have the UMI count matrix (genes by barcodes) from a mixed species experiments. The same barcode might also have few UMI counts in both human and mouse. Currently, based on few reference articles, I have got the mouse ortholog genes in human and have a data for these common genes. Essentially, two count matrix, where gene names are same and barcodes with UMI counts. How do I use this in DoubletDetection to estimate doublets. Kindly suggest. Thanks.