Issue with ligation barcode length in code

JunyueC / sci-RNA-seq3_pipeline

Processing pipeline scripts for sci-RNA-seq3 (bash, R, python)

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Issue with ligation barcode length in code #5

Open Gaura opened 3 years ago

Gaura commented 3 years ago

Given that the ligation barcode can be either 9 or 10 bp long. Wouldn't considering only 10bp length of the ligation barcode ignore all the 9bp barcode reads?

https://github.com/JunyueC/sci-RNA-seq3_pipeline/blob/f8f34970c3a5aebe87d8cce2ed22446974398cee/script_folder/UMI_barcode_attach_gzipped_with_dic.py#L44