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JustinChu / ntsm

This tools counts the number of specific k-mers within sequence data. The counts can then be compare to other counts to determine to compute the probability that sample are of the same origin to discover incongruent samples or sample swaps.
MIT License
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Sample demultiplexing #1

Closed husamia closed 4 months ago

husamia commented 10 months ago

Could I use this tool to perform sample demultiplexing? I have a set of barcode sequences and raw fastq data

JustinChu commented 10 months ago

If I understand what you need correctly, probably not. The tool looks at files as a single unit, so it doesn't really work with a mixed set of reads.

But perhaps I am unsure of your use case? What situation do you have?