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Kallepan / primer-designer

Primer Design Tool
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Amplicons longer than the region #10

Open adriandrr opened 7 months ago

adriandrr commented 7 months ago

Hi,

I had a run with primer designer with this parameters:

Screenshot 2023-11-22 at 14 58 41

Strangely, in the result, the amplicon is built over the starting point of the region. Which sequence would the pipeline take in such a case and are the primers still trustworthy?

Screenshot 2023-11-22 at 15 00 27
Kallepan commented 7 months ago

The design tool takes the region available from the given sequence. In your case the sequence before base 2.725.000 is taken as the template. This should of course be reflected in the final report...