typing additional loci - Githubissues

afadda91 commented 5 years ago

hi, when i use the -a option it only types A,B,C,DQA1,DRB1. if i specify -a MICA for instance, i get the message "Input bam : A DOES NOT exist. Please check the bam exists." I know the MICA reads are present in the KouramiPanel.bam. So what is the problem. Also, is there an explanation to the numbers that come in the results file ?

thanks

heewookl commented 5 years ago

The bam does not exist error has nothing to do with -a flag. Could you please provide the entire command line you used to run Kourami?

-a flag does not take any value. -a flag just triggers Kourami to type pre-defined additional loci: DOA DMA DMB DPA1 DPB1 DRA DRB3 DRB5 F H J L

MICA locus is currently not supported.

afadda91 commented 5 years ago

Here’s the command

java -jar ../build/Kourami.jar /Applications/kourami-0.9.6/test/0200172730_on_KouramiPanel.bam -d /Applications/kourami-0.9.6/db -o 0200172730 -a A

Input bam : A DOES NOT exist. Please check the bam exists.

Exitting . . .

When I don’t use a value for –a, it types only 5 genes: A,B,C,DQA1, DRB1.

Thanks,

From: Heewook Lee notifications@github.com<mailto:notifications@github.com> Reply-To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com> Date: Monday, November 19, 2018 at 10:26 PM To: Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org>, Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

The bam does not exist error has nothing to do with -a flag. Could you please provide the entire command line you used to run Kourami?

-a flag does not take any value. -a flag just triggers Kourami to type pre-defined additional loci: DOA DMA DMB DPA1 DPB1 DRA DRB3 DRB5 F H J L

MICA locus is currently not supported.

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heewookl commented 5 years ago

As I explained earlier '-a' flag doesn't take any value, that's why you are getting the error "A DOES NOT exist as the command-line option parser is assuming 'A' is one of input bam files.

The reason you are only getting A,B,C, DQA1, and DRB1 (Not DQB1) is probably due to an insufficient amount of sequence coverage. Are you running Kourami on whole-genome sequencing data? If so, do you know what is the average sequencing depth?

Could you also copy and paste the content of the output file ending with ".result" ? Thanks!

afadda91 commented 5 years ago

Yes its WGS of 30x. Attached is the results file.

Thanks

From: Heewook Lee notifications@github.com<mailto:notifications@github.com> Reply-To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com> Date: Tuesday, November 20, 2018 at 8:45 PM To: Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org>, Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

As I explained earlier '-a' flag doesn't take any value, that's why you are getting the error "A DOES NOT exist as the command-line option parser is assuming 'A' is one of input bam files.

The reason you are only getting A,B,C, DQA1, and DRB1 (Not DQB1) is probably due to an insufficient amount of sequence coverage. Are you running Kourami on whole-genome sequencing data? If so, do you know what is the average sequencing depth?

Could you also copy and paste the content of the output file ending with ".result" ? Thanks!

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heewookl commented 5 years ago

Could you please either copy and paste the content of the file instead of attaching the file to email? Thanks.

afadda91 commented 5 years ago

A24:02:01G 546 1.0 546 546 18.0 9.0 9.0 A24:02:01G 546 1.0 546 546 18.0 9.0 9.0 B50:01:01G 546 1.0 546 546 22.0 11.0 11.0 B50:01:01G 546 1.0 546 546 22.0 11.0 11.0 C06:02:01G 546 1.0 546 546 17.0 9.0 8.0 C06:02:01G 546 1.0 546 546 17.0 9.0 8.0 DQA105:01:01G 246 1.0 246 246 10.0 5.0 5.0 DQA105:01:01G 246 1.0 246 246 10.0 5.0 5.0 DRB103:01:01G 270 1.0 270 270 4.0 2.0 1.0 DRB103:01:01G;DRB103:01:19;DRB103:01:22;DRB103:18;DRB103:77 269 0.9962962962962963 270 270 4.0 2.0 1.0

And please, is there an explanation to the numbers?

From: Heewook Lee notifications@github.com<mailto:notifications@github.com> Reply-To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com> Date: Wednesday, November 21, 2018 at 9:26 AM To: Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org>, Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

Could you please either copy and paste the content of the file instead of attaching the file to email? Thanks.

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heewookl commented 5 years ago

They are explained in README: https://github.com/Kingsford-Group/kourami/blob/master/README.md

Last 3 columns which are depths related number and they look pretty small for 30X.

Which flavor of human reference genome did you use when you initially generated bam alignment of all WGS reads?

afadda91 commented 5 years ago

hs37

From: Heewook Lee notifications@github.com<mailto:notifications@github.com> Reply-To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com> Date: Wednesday, November 21, 2018 at 9:45 AM To: Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org>, Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

They are explained in README: https://github.com/Kingsford-Group/kourami/blob/master/README.md

Last 3 columns which are depths related number and they look pretty small for 30X.

Which flavor of human reference genome did you use when you initially generated bam alignment of all WGS reads?

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afadda91 commented 5 years ago

I meant hs38HD. I had to realign to use Kourami.

From: Heewook Lee notifications@github.com<mailto:notifications@github.com> Reply-To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com> Date: Wednesday, November 21, 2018 at 9:45 AM To: Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org>, Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

They are explained in README: https://github.com/Kingsford-Group/kourami/blob/master/README.md

Last 3 columns which are depths related number and they look pretty small for 30X.

Which flavor of human reference genome did you use when you initially generated bam alignment of all WGS reads?

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heewookl commented 5 years ago

So did you realign the entire 30x coverage of WGS to hs38HD?

Could you please run the following 2 commands? thanks!

is the baffle of the entire WGS mapped to hs38DH NOT to kourami panel. ```samtools view -H ``` ```samtools view -c ```

afadda91 commented 5 years ago

/Applications/samtools-1.9/samtools view -H /Users/afadda/Documents/MODY_family/0200172730_hs38HD_sorted.bam

@HD VN:1.6 SO:coordinate

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@SQ SN:chr5_GL949742v1_alt LN:226852

@SQ SN:chr5_KI270794v1_alt LN:164558

@SQ SN:chr5_GL339449v2_alt LN:1612928

@SQ SN:chr5_GL383530v1_alt LN:101241

@SQ SN:chr5_KI270796v1_alt LN:172708

@SQ SN:chr5_GL383531v1_alt LN:173459

@SQ SN:chr5_KI270795v1_alt LN:131892

@SQ SN:chr6_GL000250v2_alt LN:4672374

@SQ SN:chr6_KI270800v1_alt LN:175808

@SQ SN:chr6_KI270799v1_alt LN:152148

@SQ SN:chr6_GL383533v1_alt LN:124736

@SQ SN:chr6_KI270801v1_alt LN:870480

@SQ SN:chr6_KI270802v1_alt LN:75005

@SQ SN:chr6_KB021644v2_alt LN:185823

@SQ SN:chr6_KI270797v1_alt LN:197536

@SQ SN:chr6_KI270798v1_alt LN:271782

@SQ SN:chr7_KI270804v1_alt LN:157952

@SQ SN:chr7_KI270809v1_alt LN:209586

@SQ SN:chr7_KI270806v1_alt LN:158166

@SQ SN:chr7_GL383534v2_alt LN:119183

@SQ SN:chr7_KI270803v1_alt LN:1111570

@SQ SN:chr7_KI270808v1_alt LN:271455

@SQ SN:chr7_KI270807v1_alt LN:126434

@SQ SN:chr7_KI270805v1_alt LN:209988

@SQ SN:chr8_KI270818v1_alt LN:145606

@SQ SN:chr8_KI270812v1_alt LN:282736

@SQ SN:chr8_KI270811v1_alt LN:292436

@SQ SN:chr8_KI270821v1_alt LN:985506

@SQ SN:chr8_KI270813v1_alt LN:300230

@SQ SN:chr8_KI270822v1_alt LN:624492

@SQ SN:chr8_KI270814v1_alt LN:141812

@SQ SN:chr8_KI270810v1_alt LN:374415

@SQ SN:chr8_KI270819v1_alt LN:133535

@SQ SN:chr8_KI270820v1_alt LN:36640

@SQ SN:chr8_KI270817v1_alt LN:158983

@SQ SN:chr8_KI270816v1_alt LN:305841

@SQ SN:chr8_KI270815v1_alt LN:132244

@SQ SN:chr9_GL383539v1_alt LN:162988

@SQ SN:chr9_GL383540v1_alt LN:71551

@SQ SN:chr9_GL383541v1_alt LN:171286

@SQ SN:chr9_GL383542v1_alt LN:60032

@SQ SN:chr9_KI270823v1_alt LN:439082

@SQ SN:chr10_GL383545v1_alt LN:179254

@SQ SN:chr10_KI270824v1_alt LN:181496

@SQ SN:chr10_GL383546v1_alt LN:309802

@SQ SN:chr10_KI270825v1_alt LN:188315

@SQ SN:chr11_KI270832v1_alt LN:210133

@SQ SN:chr11_KI270830v1_alt LN:177092

@SQ SN:chr11_KI270831v1_alt LN:296895

@SQ SN:chr11_KI270829v1_alt LN:204059

@SQ SN:chr11_GL383547v1_alt LN:154407

@SQ SN:chr11_JH159136v1_alt LN:200998

@SQ SN:chr11_JH159137v1_alt LN:191409

@SQ SN:chr11_KI270827v1_alt LN:67707

@SQ SN:chr11_KI270826v1_alt LN:186169

@SQ SN:chr12_GL877875v1_alt LN:167313

@SQ SN:chr12_GL877876v1_alt LN:408271

@SQ SN:chr12_KI270837v1_alt LN:40090

@SQ SN:chr12_GL383549v1_alt LN:120804

@SQ SN:chr12_KI270835v1_alt LN:238139

@SQ SN:chr12_GL383550v2_alt LN:169178

@SQ SN:chr12_GL383552v1_alt LN:138655

@SQ SN:chr12_GL383553v2_alt LN:152874

@SQ SN:chr12_KI270834v1_alt LN:119498

@SQ SN:chr12_GL383551v1_alt LN:184319

@SQ SN:chr12_KI270833v1_alt LN:76061

@SQ SN:chr12_KI270836v1_alt LN:56134

@SQ SN:chr13_KI270840v1_alt LN:191684

@SQ SN:chr13_KI270839v1_alt LN:180306

@SQ SN:chr13_KI270843v1_alt LN:103832

@SQ SN:chr13_KI270841v1_alt LN:169134

@SQ SN:chr13_KI270838v1_alt LN:306913

@SQ SN:chr13_KI270842v1_alt LN:37287

@SQ SN:chr14_KI270844v1_alt LN:322166

@SQ SN:chr14_KI270847v1_alt LN:1511111

@SQ SN:chr14_KI270845v1_alt LN:180703

@SQ SN:chr14_KI270846v1_alt LN:1351393

@SQ SN:chr15_KI270852v1_alt LN:478999

@SQ SN:chr15_KI270851v1_alt LN:263054

@SQ SN:chr15_KI270848v1_alt LN:327382

@SQ SN:chr15_GL383554v1_alt LN:296527

@SQ SN:chr15_KI270849v1_alt LN:244917

@SQ SN:chr15_GL383555v2_alt LN:388773

@SQ SN:chr15_KI270850v1_alt LN:430880

@SQ SN:chr16_KI270854v1_alt LN:134193

@SQ SN:chr16_KI270856v1_alt LN:63982

@SQ SN:chr16_KI270855v1_alt LN:232857

@SQ SN:chr16_KI270853v1_alt LN:2659700

@SQ SN:chr16_GL383556v1_alt LN:192462

@SQ SN:chr16_GL383557v1_alt LN:89672

@SQ SN:chr17_GL383563v3_alt LN:375691

@SQ SN:chr17_KI270862v1_alt LN:391357

@SQ SN:chr17_KI270861v1_alt LN:196688

@SQ SN:chr17_KI270857v1_alt LN:2877074

@SQ SN:chr17_JH159146v1_alt LN:278131

@SQ SN:chr17_JH159147v1_alt LN:70345

@SQ SN:chr17_GL383564v2_alt LN:133151

@SQ SN:chr17_GL000258v2_alt LN:1821992

@SQ SN:chr17_GL383565v1_alt LN:223995

@SQ SN:chr17_KI270858v1_alt LN:235827

@SQ SN:chr17_KI270859v1_alt LN:108763

@SQ SN:chr17_GL383566v1_alt LN:90219

@SQ SN:chr17_KI270860v1_alt LN:178921

@SQ SN:chr18_KI270864v1_alt LN:111737

@SQ SN:chr18_GL383567v1_alt LN:289831

@SQ SN:chr18_GL383570v1_alt LN:164789

@SQ SN:chr18_GL383571v1_alt LN:198278

@SQ SN:chr18_GL383568v1_alt LN:104552

@SQ SN:chr18_GL383569v1_alt LN:167950

@SQ SN:chr18_GL383572v1_alt LN:159547

@SQ SN:chr18_KI270863v1_alt LN:167999

@SQ SN:chr19_KI270868v1_alt LN:61734

@SQ SN:chr19_KI270865v1_alt LN:52969

@SQ SN:chr19_GL383573v1_alt LN:385657

@SQ SN:chr19_GL383575v2_alt LN:170222

@SQ SN:chr19_GL383576v1_alt LN:188024

@SQ SN:chr19_GL383574v1_alt LN:155864

@SQ SN:chr19_KI270866v1_alt LN:43156

@SQ SN:chr19_KI270867v1_alt LN:233762

@SQ SN:chr19_GL949746v1_alt LN:987716

@SQ SN:chr20_GL383577v2_alt LN:128386

@SQ SN:chr20_KI270869v1_alt LN:118774

@SQ SN:chr20_KI270871v1_alt LN:58661

@SQ SN:chr20_KI270870v1_alt LN:183433

@SQ SN:chr21_GL383578v2_alt LN:63917

@SQ SN:chr21_KI270874v1_alt LN:166743

@SQ SN:chr21_KI270873v1_alt LN:143900

@SQ SN:chr21_GL383579v2_alt LN:201197

@SQ SN:chr21_GL383580v2_alt LN:74653

@SQ SN:chr21_GL383581v2_alt LN:116689

@SQ SN:chr21_KI270872v1_alt LN:82692

@SQ SN:chr22_KI270875v1_alt LN:259914

@SQ SN:chr22_KI270878v1_alt LN:186262

@SQ SN:chr22_KI270879v1_alt LN:304135

@SQ SN:chr22_KI270876v1_alt LN:263666

@SQ SN:chr22_KI270877v1_alt LN:101331

@SQ SN:chr22_GL383583v2_alt LN:96924

@SQ SN:chr22_GL383582v2_alt LN:162811

@SQ SN:chrX_KI270880v1_alt LN:284869

@SQ SN:chrX_KI270881v1_alt LN:144206

@SQ SN:chr19_KI270882v1_alt LN:248807

@SQ SN:chr19_KI270883v1_alt LN:170399

@SQ SN:chr19_KI270884v1_alt LN:157053

@SQ SN:chr19_KI270885v1_alt LN:171027

@SQ SN:chr19_KI270886v1_alt LN:204239

@SQ SN:chr19_KI270887v1_alt LN:209512

@SQ SN:chr19_KI270888v1_alt LN:155532

@SQ SN:chr19_KI270889v1_alt LN:170698

@SQ SN:chr19_KI270890v1_alt LN:184499

@SQ SN:chr19_KI270891v1_alt LN:170680

@SQ SN:chr1_KI270892v1_alt LN:162212

@SQ SN:chr2_KI270894v1_alt LN:214158

@SQ SN:chr2_KI270893v1_alt LN:161218

@SQ SN:chr3_KI270895v1_alt LN:162896

@SQ SN:chr4_KI270896v1_alt LN:378547

@SQ SN:chr5_KI270897v1_alt LN:1144418

@SQ SN:chr5_KI270898v1_alt LN:130957

@SQ SN:chr6_GL000251v2_alt LN:4795265

@SQ SN:chr7_KI270899v1_alt LN:190869

@SQ SN:chr8_KI270901v1_alt LN:136959

@SQ SN:chr8_KI270900v1_alt LN:318687

@SQ SN:chr11_KI270902v1_alt LN:106711

@SQ SN:chr11_KI270903v1_alt LN:214625

@SQ SN:chr12_KI270904v1_alt LN:572349

@SQ SN:chr15_KI270906v1_alt LN:196384

@SQ SN:chr15_KI270905v1_alt LN:5161414

@SQ SN:chr17_KI270907v1_alt LN:137721

@SQ SN:chr17_KI270910v1_alt LN:157099

@SQ SN:chr17_KI270909v1_alt LN:325800

@SQ SN:chr17_JH159148v1_alt LN:88070

@SQ SN:chr17_KI270908v1_alt LN:1423190

@SQ SN:chr18_KI270912v1_alt LN:174061

@SQ SN:chr18_KI270911v1_alt LN:157710

@SQ SN:chr19_GL949747v2_alt LN:729520

@SQ SN:chr22_KB663609v1_alt LN:74013

@SQ SN:chrX_KI270913v1_alt LN:274009

@SQ SN:chr19_KI270914v1_alt LN:205194

@SQ SN:chr19_KI270915v1_alt LN:170665

@SQ SN:chr19_KI270916v1_alt LN:184516

@SQ SN:chr19_KI270917v1_alt LN:190932

@SQ SN:chr19_KI270918v1_alt LN:123111

@SQ SN:chr19_KI270919v1_alt LN:170701

@SQ SN:chr19_KI270920v1_alt LN:198005

@SQ SN:chr19_KI270921v1_alt LN:282224

@SQ SN:chr19_KI270922v1_alt LN:187935

@SQ SN:chr19_KI270923v1_alt LN:189352

@SQ SN:chr3_KI270924v1_alt LN:166540

@SQ SN:chr4_KI270925v1_alt LN:555799

@SQ SN:chr6_GL000252v2_alt LN:4604811

@SQ SN:chr8_KI270926v1_alt LN:229282

@SQ SN:chr11_KI270927v1_alt LN:218612

@SQ SN:chr19_GL949748v2_alt LN:1064304

@SQ SN:chr22_KI270928v1_alt LN:176103

@SQ SN:chr19_KI270929v1_alt LN:186203

@SQ SN:chr19_KI270930v1_alt LN:200773

@SQ SN:chr19_KI270931v1_alt LN:170148

@SQ SN:chr19_KI270932v1_alt LN:215732

@SQ SN:chr19_KI270933v1_alt LN:170537

@SQ SN:chr19_GL000209v2_alt LN:177381

@SQ SN:chr3_KI270934v1_alt LN:163458

@SQ SN:chr6_GL000253v2_alt LN:4677643

@SQ SN:chr19_GL949749v2_alt LN:1091841

@SQ SN:chr3_KI270935v1_alt LN:197351

@SQ SN:chr6_GL000254v2_alt LN:4827813

@SQ SN:chr19_GL949750v2_alt LN:1066390

@SQ SN:chr3_KI270936v1_alt LN:164170

@SQ SN:chr6_GL000255v2_alt LN:4606388

@SQ SN:chr19_GL949751v2_alt LN:1002683

@SQ SN:chr3_KI270937v1_alt LN:165607

@SQ SN:chr6_GL000256v2_alt LN:4929269

@SQ SN:chr19_GL949752v1_alt LN:987100

@SQ SN:chr6_KI270758v1_alt LN:76752

@SQ SN:chr19_GL949753v2_alt LN:796479

@SQ SN:chr19_KI270938v1_alt LN:1066800

@SQ SN:chrEBV LN:171823

@SQ SN:chrUn_KN707606v1_decoy LN:2200

@SQ SN:chrUn_KN707607v1_decoy LN:3033

@SQ SN:chrUn_KN707608v1_decoy LN:3112

@SQ SN:chrUn_KN707609v1_decoy LN:1642

@SQ SN:chrUn_KN707610v1_decoy LN:1393

@SQ SN:chrUn_KN707611v1_decoy LN:1103

@SQ SN:chrUn_KN707612v1_decoy LN:1039

@SQ SN:chrUn_KN707613v1_decoy LN:1619

@SQ SN:chrUn_KN707614v1_decoy LN:3122

@SQ SN:chrUn_KN707615v1_decoy LN:1934

@SQ SN:chrUn_KN707616v1_decoy LN:3111

@SQ SN:chrUn_KN707617v1_decoy LN:2545

@SQ SN:chrUn_KN707618v1_decoy LN:2295

@SQ SN:chrUn_KN707619v1_decoy LN:1551

@SQ SN:chrUn_KN707620v1_decoy LN:2046

@SQ SN:chrUn_KN707621v1_decoy LN:1222

@SQ SN:chrUn_KN707622v1_decoy LN:1535

@SQ SN:chrUn_KN707623v1_decoy LN:3784

@SQ SN:chrUn_KN707624v1_decoy LN:1329

@SQ SN:chrUn_KN707625v1_decoy LN:1238

@SQ SN:chrUn_KN707626v1_decoy LN:5623

@SQ SN:chrUn_KN707627v1_decoy LN:5821

@SQ SN:chrUn_KN707628v1_decoy LN:2960

@SQ SN:chrUn_KN707629v1_decoy LN:1848

@SQ SN:chrUn_KN707630v1_decoy LN:2315

@SQ SN:chrUn_KN707631v1_decoy LN:1945

@SQ SN:chrUn_KN707632v1_decoy LN:1424

@SQ SN:chrUn_KN707633v1_decoy LN:1274

@SQ SN:chrUn_KN707634v1_decoy LN:1007

@SQ SN:chrUn_KN707635v1_decoy LN:1414

@SQ SN:chrUn_KN707636v1_decoy LN:1725

@SQ SN:chrUn_KN707637v1_decoy LN:5354

@SQ SN:chrUn_KN707638v1_decoy LN:2189

@SQ SN:chrUn_KN707639v1_decoy LN:1294

@SQ SN:chrUn_KN707640v1_decoy LN:1831

@SQ SN:chrUn_KN707641v1_decoy LN:1647

@SQ SN:chrUn_KN707642v1_decoy LN:2943

@SQ SN:chrUn_KN707643v1_decoy LN:2857

@SQ SN:chrUn_KN707644v1_decoy LN:1030

@SQ SN:chrUn_KN707645v1_decoy LN:1070

@SQ SN:chrUn_KN707646v1_decoy LN:1735

@SQ SN:chrUn_KN707647v1_decoy LN:1982

@SQ SN:chrUn_KN707648v1_decoy LN:1564

@SQ SN:chrUn_KN707649v1_decoy LN:1775

@SQ SN:chrUn_KN707650v1_decoy LN:1540

@SQ SN:chrUn_KN707651v1_decoy LN:2013

@SQ SN:chrUn_KN707652v1_decoy LN:1176

@SQ SN:chrUn_KN707653v1_decoy LN:1890

@SQ SN:chrUn_KN707654v1_decoy LN:3644

@SQ SN:chrUn_KN707655v1_decoy LN:2785

@SQ SN:chrUn_KN707656v1_decoy LN:1017

@SQ SN:chrUn_KN707657v1_decoy LN:1068

@SQ SN:chrUn_KN707658v1_decoy LN:1007

@SQ SN:chrUn_KN707659v1_decoy LN:2605

@SQ SN:chrUn_KN707660v1_decoy LN:8410

@SQ SN:chrUn_KN707661v1_decoy LN:5534

@SQ SN:chrUn_KN707662v1_decoy LN:2173

@SQ SN:chrUn_KN707663v1_decoy LN:1065

@SQ SN:chrUn_KN707664v1_decoy LN:8683

@SQ SN:chrUn_KN707665v1_decoy LN:2670

@SQ SN:chrUn_KN707666v1_decoy LN:2420

@SQ SN:chrUn_KN707667v1_decoy LN:2189

@SQ SN:chrUn_KN707668v1_decoy LN:2093

@SQ SN:chrUn_KN707669v1_decoy LN:1184

@SQ SN:chrUn_KN707670v1_decoy LN:1205

@SQ SN:chrUn_KN707671v1_decoy LN:2786

@SQ SN:chrUn_KN707672v1_decoy LN:2794

@SQ SN:chrUn_KN707673v1_decoy LN:19544

@SQ SN:chrUn_KN707674v1_decoy LN:2848

@SQ SN:chrUn_KN707675v1_decoy LN:10556

@SQ SN:chrUn_KN707676v1_decoy LN:9066

@SQ SN:chrUn_KN707677v1_decoy LN:7267

@SQ SN:chrUn_KN707678v1_decoy LN:2462

@SQ SN:chrUn_KN707679v1_decoy LN:1774

@SQ SN:chrUn_KN707680v1_decoy LN:1297

@SQ SN:chrUn_KN707681v1_decoy LN:4379

@SQ SN:chrUn_KN707682v1_decoy LN:4208

@SQ SN:chrUn_KN707683v1_decoy LN:4068

@SQ SN:chrUn_KN707684v1_decoy LN:2940

@SQ SN:chrUn_KN707685v1_decoy LN:3938

@SQ SN:chrUn_KN707686v1_decoy LN:2072

@SQ SN:chrUn_KN707687v1_decoy LN:1136

@SQ SN:chrUn_KN707688v1_decoy LN:4248

@SQ SN:chrUn_KN707689v1_decoy LN:5823

@SQ SN:chrUn_KN707690v1_decoy LN:3715

@SQ SN:chrUn_KN707691v1_decoy LN:4885

@SQ SN:chrUn_KN707692v1_decoy LN:4813

@SQ SN:chrUn_KN707693v1_decoy LN:2899

@SQ SN:chrUn_KN707694v1_decoy LN:1228

@SQ SN:chrUn_KN707695v1_decoy LN:3119

@SQ SN:chrUn_KN707696v1_decoy LN:3828

@SQ SN:chrUn_KN707697v1_decoy LN:1186

@SQ SN:chrUn_KN707698v1_decoy LN:1908

@SQ SN:chrUn_KN707699v1_decoy LN:2795

@SQ SN:chrUn_KN707700v1_decoy LN:3703

@SQ SN:chrUn_KN707701v1_decoy LN:6722

@SQ SN:chrUn_KN707702v1_decoy LN:6466

@SQ SN:chrUn_KN707703v1_decoy LN:2235

@SQ SN:chrUn_KN707704v1_decoy LN:2871

@SQ SN:chrUn_KN707705v1_decoy LN:4632

@SQ SN:chrUn_KN707706v1_decoy LN:4225

@SQ SN:chrUn_KN707707v1_decoy LN:4339

@SQ SN:chrUn_KN707708v1_decoy LN:2305

@SQ SN:chrUn_KN707709v1_decoy LN:3273

@SQ SN:chrUn_KN707710v1_decoy LN:5701

@SQ SN:chrUn_KN707711v1_decoy LN:4154

@SQ SN:chrUn_KN707712v1_decoy LN:1243

@SQ SN:chrUn_KN707713v1_decoy LN:1308

@SQ SN:chrUn_KN707714v1_decoy LN:2922

@SQ SN:chrUn_KN707715v1_decoy LN:3044

@SQ SN:chrUn_KN707716v1_decoy LN:2888

@SQ SN:chrUn_KN707717v1_decoy LN:1742

@SQ SN:chrUn_KN707718v1_decoy LN:4969

@SQ SN:chrUn_KN707719v1_decoy LN:3270

@SQ SN:chrUn_KN707720v1_decoy LN:6028

@SQ SN:chrUn_KN707721v1_decoy LN:1105

@SQ SN:chrUn_KN707722v1_decoy LN:2884

@SQ SN:chrUn_KN707723v1_decoy LN:1124

@SQ SN:chrUn_KN707724v1_decoy LN:1454

@SQ SN:chrUn_KN707725v1_decoy LN:2565

@SQ SN:chrUn_KN707726v1_decoy LN:2149

@SQ SN:chrUn_KN707727v1_decoy LN:2630

@SQ SN:chrUn_KN707728v1_decoy LN:14625

@SQ SN:chrUn_KN707729v1_decoy LN:7431

@SQ SN:chrUn_KN707730v1_decoy LN:5776

@SQ SN:chrUn_KN707731v1_decoy LN:4820

@SQ SN:chrUn_KN707732v1_decoy LN:1227

@SQ SN:chrUn_KN707733v1_decoy LN:7503

@SQ SN:chrUn_KN707734v1_decoy LN:9652

@SQ SN:chrUn_KN707735v1_decoy LN:1091

@SQ SN:chrUn_KN707736v1_decoy LN:2467

@SQ SN:chrUn_KN707737v1_decoy LN:1270

@SQ SN:chrUn_KN707738v1_decoy LN:4365

@SQ SN:chrUn_KN707739v1_decoy LN:4284

@SQ SN:chrUn_KN707740v1_decoy LN:10282

@SQ SN:chrUn_KN707741v1_decoy LN:5601

@SQ SN:chrUn_KN707742v1_decoy LN:4758

@SQ SN:chrUn_KN707743v1_decoy LN:1624

@SQ SN:chrUn_KN707744v1_decoy LN:4024

@SQ SN:chrUn_KN707745v1_decoy LN:1276

@SQ SN:chrUn_KN707746v1_decoy LN:5083

@SQ SN:chrUn_KN707747v1_decoy LN:2075

@SQ SN:chrUn_KN707748v1_decoy LN:3553

@SQ SN:chrUn_KN707749v1_decoy LN:7010

@SQ SN:chrUn_KN707750v1_decoy LN:4718

@SQ SN:chrUn_KN707751v1_decoy LN:3546

@SQ SN:chrUn_KN707752v1_decoy LN:2873

@SQ SN:chrUn_KN707753v1_decoy LN:2144

@SQ SN:chrUn_KN707754v1_decoy LN:2243

@SQ SN:chrUn_KN707755v1_decoy LN:5343

@SQ SN:chrUn_KN707756v1_decoy LN:4877

@SQ SN:chrUn_KN707757v1_decoy LN:3034

@SQ SN:chrUn_KN707758v1_decoy LN:2826

@SQ SN:chrUn_KN707759v1_decoy LN:1221

@SQ SN:chrUn_KN707760v1_decoy LN:1169

@SQ SN:chrUn_KN707761v1_decoy LN:2319

@SQ SN:chrUn_KN707762v1_decoy LN:3450

@SQ SN:chrUn_KN707763v1_decoy LN:2674

@SQ SN:chrUn_KN707764v1_decoy LN:3912

@SQ SN:chrUn_KN707765v1_decoy LN:6020

@SQ SN:chrUn_KN707766v1_decoy LN:2303

@SQ SN:chrUn_KN707767v1_decoy LN:2552

@SQ SN:chrUn_KN707768v1_decoy LN:3656

@SQ SN:chrUn_KN707769v1_decoy LN:1591

@SQ SN:chrUn_KN707770v1_decoy LN:1209

@SQ SN:chrUn_KN707771v1_decoy LN:3176

@SQ SN:chrUn_KN707772v1_decoy LN:8915

@SQ SN:chrUn_KN707773v1_decoy LN:4902

@SQ SN:chrUn_KN707774v1_decoy LN:3324

@SQ SN:chrUn_KN707775v1_decoy LN:5997

@SQ SN:chrUn_KN707776v1_decoy LN:2618

@SQ SN:chrUn_KN707777v1_decoy LN:10311

@SQ SN:chrUn_KN707778v1_decoy LN:2440

@SQ SN:chrUn_KN707779v1_decoy LN:12444

@SQ SN:chrUn_KN707780v1_decoy LN:5691

@SQ SN:chrUn_KN707781v1_decoy LN:2717

@SQ SN:chrUn_KN707782v1_decoy LN:5277

@SQ SN:chrUn_KN707783v1_decoy LN:4373

@SQ SN:chrUn_KN707784v1_decoy LN:3224

@SQ SN:chrUn_KN707785v1_decoy LN:2631

@SQ SN:chrUn_KN707786v1_decoy LN:5385

@SQ SN:chrUn_KN707787v1_decoy LN:3678

@SQ SN:chrUn_KN707788v1_decoy LN:1412

@SQ SN:chrUn_KN707789v1_decoy LN:1443

@SQ SN:chrUn_KN707790v1_decoy LN:1098

@SQ SN:chrUn_KN707791v1_decoy LN:3240

@SQ SN:chrUn_KN707792v1_decoy LN:1915

@SQ SN:chrUn_KN707793v1_decoy LN:4667

@SQ SN:chrUn_KN707794v1_decoy LN:7219

@SQ SN:chrUn_KN707795v1_decoy LN:3277

@SQ SN:chrUn_KN707796v1_decoy LN:3473

@SQ SN:chrUn_KN707797v1_decoy LN:4243

@SQ SN:chrUn_KN707798v1_decoy LN:17599

@SQ SN:chrUn_KN707799v1_decoy LN:5095

@SQ SN:chrUn_KN707800v1_decoy LN:2237

@SQ SN:chrUn_KN707801v1_decoy LN:2901

@SQ SN:chrUn_KN707802v1_decoy LN:2666

@SQ SN:chrUn_KN707803v1_decoy LN:5336

@SQ SN:chrUn_KN707804v1_decoy LN:4383

@SQ SN:chrUn_KN707805v1_decoy LN:5446

@SQ SN:chrUn_KN707806v1_decoy LN:6252

@SQ SN:chrUn_KN707807v1_decoy LN:4616

@SQ SN:chrUn_KN707808v1_decoy LN:3021

@SQ SN:chrUn_KN707809v1_decoy LN:3667

@SQ SN:chrUn_KN707810v1_decoy LN:4563

@SQ SN:chrUn_KN707811v1_decoy LN:1120

@SQ SN:chrUn_KN707812v1_decoy LN:3845

@SQ SN:chrUn_KN707813v1_decoy LN:2272

@SQ SN:chrUn_KN707814v1_decoy LN:4764

@SQ SN:chrUn_KN707815v1_decoy LN:5410

@SQ SN:chrUn_KN707816v1_decoy LN:7150

@SQ SN:chrUn_KN707817v1_decoy LN:1762

@SQ SN:chrUn_KN707818v1_decoy LN:1207

@SQ SN:chrUn_KN707819v1_decoy LN:1331

@SQ SN:chrUn_KN707820v1_decoy LN:8307

@SQ SN:chrUn_KN707821v1_decoy LN:2276

@SQ SN:chrUn_KN707822v1_decoy LN:2575

@SQ SN:chrUn_KN707823v1_decoy LN:3970

@SQ SN:chrUn_KN707824v1_decoy LN:1352

@SQ SN:chrUn_KN707825v1_decoy LN:3040

@SQ SN:chrUn_KN707826v1_decoy LN:2070

@SQ SN:chrUn_KN707827v1_decoy LN:2913

@SQ SN:chrUn_KN707828v1_decoy LN:2389

@SQ SN:chrUn_KN707829v1_decoy LN:1835

@SQ SN:chrUn_KN707830v1_decoy LN:4807

@SQ SN:chrUn_KN707831v1_decoy LN:2201

@SQ SN:chrUn_KN707832v1_decoy LN:1265

@SQ SN:chrUn_KN707833v1_decoy LN:1961

@SQ SN:chrUn_KN707834v1_decoy LN:1064

@SQ SN:chrUn_KN707835v1_decoy LN:1932

@SQ SN:chrUn_KN707836v1_decoy LN:3213

@SQ SN:chrUn_KN707837v1_decoy LN:1178

@SQ SN:chrUn_KN707838v1_decoy LN:2926

@SQ SN:chrUn_KN707839v1_decoy LN:1038

@SQ SN:chrUn_KN707840v1_decoy LN:3298

@SQ SN:chrUn_KN707841v1_decoy LN:8992

@SQ SN:chrUn_KN707842v1_decoy LN:6698

@SQ SN:chrUn_KN707843v1_decoy LN:4880

@SQ SN:chrUn_KN707844v1_decoy LN:1766

@SQ SN:chrUn_KN707845v1_decoy LN:3532

@SQ SN:chrUn_KN707846v1_decoy LN:2297

@SQ SN:chrUn_KN707847v1_decoy LN:1234

@SQ SN:chrUn_KN707848v1_decoy LN:1205

@SQ SN:chrUn_KN707849v1_decoy LN:2790

@SQ SN:chrUn_KN707850v1_decoy LN:2006

@SQ SN:chrUn_KN707851v1_decoy LN:4593

@SQ SN:chrUn_KN707852v1_decoy LN:1579

@SQ SN:chrUn_KN707853v1_decoy LN:9597

@SQ SN:chrUn_KN707854v1_decoy LN:10451

@SQ SN:chrUn_KN707855v1_decoy LN:3219

@SQ SN:chrUn_KN707856v1_decoy LN:2300

@SQ SN:chrUn_KN707857v1_decoy LN:5985

@SQ SN:chrUn_KN707858v1_decoy LN:2959

@SQ SN:chrUn_KN707859v1_decoy LN:1340

@SQ SN:chrUn_KN707860v1_decoy LN:3148

@SQ SN:chrUn_KN707861v1_decoy LN:2242

@SQ SN:chrUn_KN707862v1_decoy LN:16513

@SQ SN:chrUn_KN707863v1_decoy LN:7821

@SQ SN:chrUn_KN707864v1_decoy LN:2159

@SQ SN:chrUn_KN707865v1_decoy LN:2114

@SQ SN:chrUn_KN707866v1_decoy LN:4109

@SQ SN:chrUn_KN707867v1_decoy LN:1544

@SQ SN:chrUn_KN707868v1_decoy LN:1005

@SQ SN:chrUn_KN707869v1_decoy LN:8632

@SQ SN:chrUn_KN707870v1_decoy LN:1012

@SQ SN:chrUn_KN707871v1_decoy LN:4728

@SQ SN:chrUn_KN707872v1_decoy LN:2165

@SQ SN:chrUn_KN707873v1_decoy LN:7591

@SQ SN:chrUn_KN707874v1_decoy LN:5202

@SQ SN:chrUn_KN707875v1_decoy LN:4241

@SQ SN:chrUn_KN707876v1_decoy LN:4131

@SQ SN:chrUn_KN707877v1_decoy LN:2272

@SQ SN:chrUn_KN707878v1_decoy LN:2085

@SQ SN:chrUn_KN707879v1_decoy LN:4346

@SQ SN:chrUn_KN707880v1_decoy LN:1208

@SQ SN:chrUn_KN707881v1_decoy LN:4543

@SQ SN:chrUn_KN707882v1_decoy LN:2772

@SQ SN:chrUn_KN707883v1_decoy LN:2490

@SQ SN:chrUn_KN707884v1_decoy LN:4568

@SQ SN:chrUn_KN707885v1_decoy LN:1776

@SQ SN:chrUn_KN707886v1_decoy LN:2699

@SQ SN:chrUn_KN707887v1_decoy LN:3534

@SQ SN:chrUn_KN707888v1_decoy LN:2424

@SQ SN:chrUn_KN707889v1_decoy LN:1747

@SQ SN:chrUn_KN707890v1_decoy LN:1088

@SQ SN:chrUn_KN707891v1_decoy LN:1143

@SQ SN:chrUn_KN707892v1_decoy LN:2530

@SQ SN:chrUn_KN707893v1_decoy LN:8049

@SQ SN:chrUn_KN707894v1_decoy LN:1366

@SQ SN:chrUn_KN707895v1_decoy LN:4284

@SQ SN:chrUn_KN707896v1_decoy LN:33125

@SQ SN:chrUn_KN707897v1_decoy LN:2137

@SQ SN:chrUn_KN707898v1_decoy LN:3840

@SQ SN:chrUn_KN707899v1_decoy LN:3087

@SQ SN:chrUn_KN707900v1_decoy LN:2041

@SQ SN:chrUn_KN707901v1_decoy LN:3344

@SQ SN:chrUn_KN707902v1_decoy LN:2921

@SQ SN:chrUn_KN707903v1_decoy LN:6581

@SQ SN:chrUn_KN707904v1_decoy LN:3968

@SQ SN:chrUn_KN707905v1_decoy LN:2339

@SQ SN:chrUn_KN707906v1_decoy LN:1243

@SQ SN:chrUn_KN707907v1_decoy LN:7776

@SQ SN:chrUn_KN707908v1_decoy LN:19837

@SQ SN:chrUn_KN707909v1_decoy LN:1737

@SQ SN:chrUn_KN707910v1_decoy LN:1098

@SQ SN:chrUn_KN707911v1_decoy LN:1893

@SQ SN:chrUn_KN707912v1_decoy LN:1281

@SQ SN:chrUn_KN707913v1_decoy LN:1527

@SQ SN:chrUn_KN707914v1_decoy LN:2055

@SQ SN:chrUn_KN707915v1_decoy LN:2527

@SQ SN:chrUn_KN707916v1_decoy LN:3275

@SQ SN:chrUn_KN707917v1_decoy LN:1265

@SQ SN:chrUn_KN707918v1_decoy LN:2623

@SQ SN:chrUn_KN707919v1_decoy LN:4850

@SQ SN:chrUn_KN707920v1_decoy LN:3584

@SQ SN:chrUn_KN707921v1_decoy LN:2561

@SQ SN:chrUn_KN707922v1_decoy LN:3041

@SQ SN:chrUn_KN707923v1_decoy LN:1409

@SQ SN:chrUn_KN707924v1_decoy LN:4596

@SQ SN:chrUn_KN707925v1_decoy LN:11555

@SQ SN:chrUn_KN707926v1_decoy LN:1266

@SQ SN:chrUn_KN707927v1_decoy LN:1079

@SQ SN:chrUn_KN707928v1_decoy LN:1087

@SQ SN:chrUn_KN707929v1_decoy LN:1226

@SQ SN:chrUn_KN707930v1_decoy LN:1131

@SQ SN:chrUn_KN707931v1_decoy LN:1199

@SQ SN:chrUn_KN707932v1_decoy LN:1084

@SQ SN:chrUn_KN707933v1_decoy LN:2038

@SQ SN:chrUn_KN707934v1_decoy LN:1070

@SQ SN:chrUn_KN707935v1_decoy LN:1312

@SQ SN:chrUn_KN707936v1_decoy LN:4031

@SQ SN:chrUn_KN707937v1_decoy LN:7445

@SQ SN:chrUn_KN707938v1_decoy LN:1770

@SQ SN:chrUn_KN707939v1_decoy LN:5600

@SQ SN:chrUn_KN707940v1_decoy LN:1882

@SQ SN:chrUn_KN707941v1_decoy LN:1170

@SQ SN:chrUn_KN707942v1_decoy LN:1300

@SQ SN:chrUn_KN707943v1_decoy LN:5325

@SQ SN:chrUn_KN707944v1_decoy LN:2043

@SQ SN:chrUn_KN707945v1_decoy LN:1072

@SQ SN:chrUn_KN707946v1_decoy LN:2463

@SQ SN:chrUn_KN707947v1_decoy LN:1010

@SQ SN:chrUn_KN707948v1_decoy LN:1432

@SQ SN:chrUn_KN707949v1_decoy LN:1162

@SQ SN:chrUn_KN707950v1_decoy LN:1095

@SQ SN:chrUn_KN707951v1_decoy LN:1118

@SQ SN:chrUn_KN707952v1_decoy LN:1383

@SQ SN:chrUn_KN707953v1_decoy LN:2289

@SQ SN:chrUn_KN707954v1_decoy LN:1648

@SQ SN:chrUn_KN707955v1_decoy LN:2203

@SQ SN:chrUn_KN707956v1_decoy LN:3270

@SQ SN:chrUn_KN707957v1_decoy LN:11499

@SQ SN:chrUn_KN707958v1_decoy LN:2474

@SQ SN:chrUn_KN707959v1_decoy LN:2294

@SQ SN:chrUn_KN707960v1_decoy LN:1238

@SQ SN:chrUn_KN707961v1_decoy LN:3410

@SQ SN:chrUn_KN707962v1_decoy LN:1523

@SQ SN:chrUn_KN707963v1_decoy LN:62955

@SQ SN:chrUn_KN707964v1_decoy LN:6282

@SQ SN:chrUn_KN707965v1_decoy LN:3836

@SQ SN:chrUn_KN707966v1_decoy LN:6486

@SQ SN:chrUn_KN707967v1_decoy LN:15368

@SQ SN:chrUn_KN707968v1_decoy LN:9572

@SQ SN:chrUn_KN707969v1_decoy LN:6413

@SQ SN:chrUn_KN707970v1_decoy LN:4104

@SQ SN:chrUn_KN707971v1_decoy LN:12943

@SQ SN:chrUn_KN707972v1_decoy LN:4650

@SQ SN:chrUn_KN707973v1_decoy LN:3080

@SQ SN:chrUn_KN707974v1_decoy LN:3134

@SQ SN:chrUn_KN707975v1_decoy LN:6211

@SQ SN:chrUn_KN707976v1_decoy LN:1126

@SQ SN:chrUn_KN707977v1_decoy LN:1101

@SQ SN:chrUn_KN707978v1_decoy LN:1101

@SQ SN:chrUn_KN707979v1_decoy LN:2648

@SQ SN:chrUn_KN707980v1_decoy LN:2973

@SQ SN:chrUn_KN707981v1_decoy LN:2520

@SQ SN:chrUn_KN707982v1_decoy LN:2318

@SQ SN:chrUn_KN707983v1_decoy LN:2606

@SQ SN:chrUn_KN707984v1_decoy LN:2205

@SQ SN:chrUn_KN707985v1_decoy LN:2929

@SQ SN:chrUn_KN707986v1_decoy LN:3869

@SQ SN:chrUn_KN707987v1_decoy LN:1117

@SQ SN:chrUn_KN707988v1_decoy LN:2960

@SQ SN:chrUn_KN707989v1_decoy LN:1009

@SQ SN:chrUn_KN707990v1_decoy LN:4048

@SQ SN:chrUn_KN707991v1_decoy LN:2193

@SQ SN:chrUn_KN707992v1_decoy LN:1830

@SQ SN:chrUn_JTFH01000001v1_decoy LN:25139

@SQ SN:chrUn_JTFH01000002v1_decoy LN:18532

@SQ SN:chrUn_JTFH01000003v1_decoy LN:15240

@SQ SN:chrUn_JTFH01000004v1_decoy LN:13739

@SQ SN:chrUn_JTFH01000005v1_decoy LN:11297

@SQ SN:chrUn_JTFH01000006v1_decoy LN:10074

@SQ SN:chrUn_JTFH01000007v1_decoy LN:9891

@SQ SN:chrUn_JTFH01000008v1_decoy LN:9774

@SQ SN:chrUn_JTFH01000009v1_decoy LN:9727

@SQ SN:chrUn_JTFH01000010v1_decoy LN:9358

@SQ SN:chrUn_JTFH01000011v1_decoy LN:8920

@SQ SN:chrUn_JTFH01000012v1_decoy LN:8479

@SQ SN:chrUn_JTFH01000013v1_decoy LN:8312

@SQ SN:chrUn_JTFH01000014v1_decoy LN:8261

@SQ SN:chrUn_JTFH01000015v1_decoy LN:8131

@SQ SN:chrUn_JTFH01000016v1_decoy LN:8051

@SQ SN:chrUn_JTFH01000017v1_decoy LN:7832

@SQ SN:chrUn_JTFH01000018v1_decoy LN:7710

@SQ SN:chrUn_JTFH01000019v1_decoy LN:7702

@SQ SN:chrUn_JTFH01000020v1_decoy LN:7479

@SQ SN:chrUn_JTFH01000021v1_decoy LN:7368

@SQ SN:chrUn_JTFH01000022v1_decoy LN:7162

@SQ SN:chrUn_JTFH01000023v1_decoy LN:7065

@SQ SN:chrUn_JTFH01000024v1_decoy LN:7019

@SQ SN:chrUn_JTFH01000025v1_decoy LN:6997

@SQ SN:chrUn_JTFH01000026v1_decoy LN:6994

@SQ SN:chrUn_JTFH01000027v1_decoy LN:6979

@SQ SN:chrUn_JTFH01000028v1_decoy LN:6797

@SQ SN:chrUn_JTFH01000029v1_decoy LN:6525

@SQ SN:chrUn_JTFH01000030v1_decoy LN:6246

@SQ SN:chrUn_JTFH01000031v1_decoy LN:5926

@SQ SN:chrUn_JTFH01000032v1_decoy LN:5914

@SQ SN:chrUn_JTFH01000033v1_decoy LN:5898

@SQ SN:chrUn_JTFH01000034v1_decoy LN:5879

@SQ SN:chrUn_JTFH01000035v1_decoy LN:5834

@SQ SN:chrUn_JTFH01000036v1_decoy LN:5743

@SQ SN:chrUn_JTFH01000037v1_decoy LN:5577

@SQ SN:chrUn_JTFH01000038v1_decoy LN:5413

@SQ SN:chrUn_JTFH01000039v1_decoy LN:5250

@SQ SN:chrUn_JTFH01000040v1_decoy LN:5246

@SQ SN:chrUn_JTFH01000041v1_decoy LN:5118

@SQ SN:chrUn_JTFH01000042v1_decoy LN:5058

@SQ SN:chrUn_JTFH01000043v1_decoy LN:4959

@SQ SN:chrUn_JTFH01000044v1_decoy LN:4853

@SQ SN:chrUn_JTFH01000045v1_decoy LN:4828

@SQ SN:chrUn_JTFH01000046v1_decoy LN:4819

@SQ SN:chrUn_JTFH01000047v1_decoy LN:4809

@SQ SN:chrUn_JTFH01000048v1_decoy LN:4710

@SQ SN:chrUn_JTFH01000049v1_decoy LN:4680

@SQ SN:chrUn_JTFH01000050v1_decoy LN:4645

@SQ SN:chrUn_JTFH01000051v1_decoy LN:4514

@SQ SN:chrUn_JTFH01000052v1_decoy LN:4439

@SQ SN:chrUn_JTFH01000053v1_decoy LN:4416

@SQ SN:chrUn_JTFH01000054v1_decoy LN:4409

@SQ SN:chrUn_JTFH01000055v1_decoy LN:4392

@SQ SN:chrUn_JTFH01000056v1_decoy LN:4359

@SQ SN:chrUn_JTFH01000057v1_decoy LN:4319

@SQ SN:chrUn_JTFH01000058v1_decoy LN:4290

@SQ SN:chrUn_JTFH01000059v1_decoy LN:4242

@SQ SN:chrUn_JTFH01000060v1_decoy LN:4228

@SQ SN:chrUn_JTFH01000061v1_decoy LN:4222

@SQ SN:chrUn_JTFH01000062v1_decoy LN:4216

@SQ SN:chrUn_JTFH01000063v1_decoy LN:4210

@SQ SN:chrUn_JTFH01000064v1_decoy LN:4206

@SQ SN:chrUn_JTFH01000065v1_decoy LN:4102

@SQ SN:chrUn_JTFH01000066v1_decoy LN:4101

@SQ SN:chrUn_JTFH01000067v1_decoy LN:4083

@SQ SN:chrUn_JTFH01000068v1_decoy LN:3967

@SQ SN:chrUn_JTFH01000069v1_decoy LN:3955

@SQ SN:chrUn_JTFH01000070v1_decoy LN:3945

@SQ SN:chrUn_JTFH01000071v1_decoy LN:3930

@SQ SN:chrUn_JTFH01000072v1_decoy LN:3929

@SQ SN:chrUn_JTFH01000073v1_decoy LN:3924

@SQ SN:chrUn_JTFH01000074v1_decoy LN:3919

@SQ SN:chrUn_JTFH01000075v1_decoy LN:3908

@SQ SN:chrUn_JTFH01000076v1_decoy LN:3892

@SQ SN:chrUn_JTFH01000077v1_decoy LN:3890

@SQ SN:chrUn_JTFH01000078v1_decoy LN:3859

@SQ SN:chrUn_JTFH01000079v1_decoy LN:3846

@SQ SN:chrUn_JTFH01000080v1_decoy LN:3835

@SQ SN:chrUn_JTFH01000081v1_decoy LN:3830

@SQ SN:chrUn_JTFH01000082v1_decoy LN:3828

@SQ SN:chrUn_JTFH01000083v1_decoy LN:3825

@SQ SN:chrUn_JTFH01000084v1_decoy LN:3821

@SQ SN:chrUn_JTFH01000085v1_decoy LN:3809

@SQ SN:chrUn_JTFH01000086v1_decoy LN:3801

@SQ SN:chrUn_JTFH01000087v1_decoy LN:3799

@SQ SN:chrUn_JTFH01000088v1_decoy LN:3737

@SQ SN:chrUn_JTFH01000089v1_decoy LN:3701

@SQ SN:chrUn_JTFH01000090v1_decoy LN:3698

@SQ SN:chrUn_JTFH01000091v1_decoy LN:3692

@SQ SN:chrUn_JTFH01000092v1_decoy LN:3686

@SQ SN:chrUn_JTFH01000093v1_decoy LN:3677

@SQ SN:chrUn_JTFH01000094v1_decoy LN:3664

@SQ SN:chrUn_JTFH01000095v1_decoy LN:3613

@SQ SN:chrUn_JTFH01000096v1_decoy LN:3611

@SQ SN:chrUn_JTFH01000097v1_decoy LN:3606

@SQ SN:chrUn_JTFH01000098v1_decoy LN:3584

@SQ SN:chrUn_JTFH01000099v1_decoy LN:3581

@SQ SN:chrUn_JTFH01000100v1_decoy LN:3543

@SQ SN:chrUn_JTFH01000101v1_decoy LN:3528

@SQ SN:chrUn_JTFH01000102v1_decoy LN:3527

@SQ SN:chrUn_JTFH01000103v1_decoy LN:3496

@SQ SN:chrUn_JTFH01000104v1_decoy LN:3493

@SQ SN:chrUn_JTFH01000105v1_decoy LN:3484

@SQ SN:chrUn_JTFH01000106v1_decoy LN:3435

@SQ SN:chrUn_JTFH01000107v1_decoy LN:3391

@SQ SN:chrUn_JTFH01000108v1_decoy LN:3374

@SQ SN:chrUn_JTFH01000109v1_decoy LN:3371

@SQ SN:chrUn_JTFH01000110v1_decoy LN:3361

@SQ SN:chrUn_JTFH01000111v1_decoy LN:3351

@SQ SN:chrUn_JTFH01000112v1_decoy LN:3340

@SQ SN:chrUn_JTFH01000113v1_decoy LN:3320

@SQ SN:chrUn_JTFH01000114v1_decoy LN:3282

@SQ SN:chrUn_JTFH01000115v1_decoy LN:3278

@SQ SN:chrUn_JTFH01000116v1_decoy LN:3260

@SQ SN:chrUn_JTFH01000117v1_decoy LN:3258

@SQ SN:chrUn_JTFH01000118v1_decoy LN:3253

@SQ SN:chrUn_JTFH01000119v1_decoy LN:3247

@SQ SN:chrUn_JTFH01000120v1_decoy LN:3230

@SQ SN:chrUn_JTFH01000121v1_decoy LN:3224

@SQ SN:chrUn_JTFH01000122v1_decoy LN:3216

@SQ SN:chrUn_JTFH01000123v1_decoy LN:3212

@SQ SN:chrUn_JTFH01000124v1_decoy LN:3194

@SQ SN:chrUn_JTFH01000125v1_decoy LN:3189

@SQ SN:chrUn_JTFH01000126v1_decoy LN:3177

@SQ SN:chrUn_JTFH01000127v1_decoy LN:3176

@SQ SN:chrUn_JTFH01000128v1_decoy LN:3173

@SQ SN:chrUn_JTFH01000129v1_decoy LN:3170

@SQ SN:chrUn_JTFH01000130v1_decoy LN:3166

@SQ SN:chrUn_JTFH01000131v1_decoy LN:3163

@SQ SN:chrUn_JTFH01000132v1_decoy LN:3143

@SQ SN:chrUn_JTFH01000133v1_decoy LN:3137

@SQ SN:chrUn_JTFH01000134v1_decoy LN:3116

@SQ SN:chrUn_JTFH01000135v1_decoy LN:3106

@SQ SN:chrUn_JTFH01000136v1_decoy LN:3093

@SQ SN:chrUn_JTFH01000137v1_decoy LN:3079

@SQ SN:chrUn_JTFH01000138v1_decoy LN:3053

@SQ SN:chrUn_JTFH01000139v1_decoy LN:3051

@SQ SN:chrUn_JTFH01000140v1_decoy LN:3015

@SQ SN:chrUn_JTFH01000141v1_decoy LN:3012

@SQ SN:chrUn_JTFH01000142v1_decoy LN:3009

@SQ SN:chrUn_JTFH01000143v1_decoy LN:2997

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@SQ SN:chrUn_JTFH01000163v1_decoy LN:2859

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@SQ SN:chrUn_JTFH01000300v1_decoy LN:2134

@SQ SN:chrUn_JTFH01000301v1_decoy LN:2129

@SQ SN:chrUn_JTFH01000302v1_decoy LN:2128

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@SQ SN:chrUn_JTFH01000308v1_decoy LN:2094

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@SQ SN:chrUn_JTFH01000321v1_decoy LN:2053

@SQ SN:chrUn_JTFH01000322v1_decoy LN:2040

@SQ SN:chrUn_JTFH01000323v1_decoy LN:2036

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@SQ SN:chrUn_JTFH01000325v1_decoy LN:2034

@SQ SN:chrUn_JTFH01000326v1_decoy LN:2032

@SQ SN:chrUn_JTFH01000327v1_decoy LN:2029

@SQ SN:chrUn_JTFH01000328v1_decoy LN:2025

@SQ SN:chrUn_JTFH01000329v1_decoy LN:2021

@SQ SN:chrUn_JTFH01000330v1_decoy LN:2018

@SQ SN:chrUn_JTFH01000331v1_decoy LN:2015

@SQ SN:chrUn_JTFH01000332v1_decoy LN:2009

@SQ SN:chrUn_JTFH01000333v1_decoy LN:2007

@SQ SN:chrUn_JTFH01000334v1_decoy LN:2005

@SQ SN:chrUn_JTFH01000335v1_decoy LN:2003

@SQ SN:chrUn_JTFH01000336v1_decoy LN:2001

@SQ SN:chrUn_JTFH01000337v1_decoy LN:2001

@SQ SN:chrUn_JTFH01000338v1_decoy LN:2000

@SQ SN:chrUn_JTFH01000339v1_decoy LN:1996

@SQ SN:chrUn_JTFH01000340v1_decoy LN:1992

@SQ SN:chrUn_JTFH01000341v1_decoy LN:1985

@SQ SN:chrUn_JTFH01000342v1_decoy LN:1981

@SQ SN:chrUn_JTFH01000343v1_decoy LN:1977

@SQ SN:chrUn_JTFH01000344v1_decoy LN:1971

@SQ SN:chrUn_JTFH01000345v1_decoy LN:1968

@SQ SN:chrUn_JTFH01000346v1_decoy LN:1962

@SQ SN:chrUn_JTFH01000347v1_decoy LN:1961

@SQ SN:chrUn_JTFH01000348v1_decoy LN:1960

@SQ SN:chrUn_JTFH01000349v1_decoy LN:1960

@SQ SN:chrUn_JTFH01000350v1_decoy LN:1954

@SQ SN:chrUn_JTFH01000351v1_decoy LN:1952

@SQ SN:chrUn_JTFH01000352v1_decoy LN:1947

@SQ SN:chrUn_JTFH01000353v1_decoy LN:1944

@SQ SN:chrUn_JTFH01000354v1_decoy LN:1943

@SQ SN:chrUn_JTFH01000355v1_decoy LN:1941

@SQ SN:chrUn_JTFH01000356v1_decoy LN:1937

@SQ SN:chrUn_JTFH01000357v1_decoy LN:1934

@SQ SN:chrUn_JTFH01000358v1_decoy LN:1929

@SQ SN:chrUn_JTFH01000359v1_decoy LN:1924

@SQ SN:chrUn_JTFH01000360v1_decoy LN:1924

@SQ SN:chrUn_JTFH01000361v1_decoy LN:1923

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@SQ SN:chrUn_JTFH01000363v1_decoy LN:1918

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@SQ SN:chrUn_JTFH01000480v1_decoy LN:1624

@SQ SN:chrUn_JTFH01000481v1_decoy LN:1617

@SQ SN:chrUn_JTFH01000482v1_decoy LN:1616

@SQ SN:chrUn_JTFH01000483v1_decoy LN:1615

@SQ SN:chrUn_JTFH01000484v1_decoy LN:1611

@SQ SN:chrUn_JTFH01000485v1_decoy LN:1611

@SQ SN:chrUn_JTFH01000486v1_decoy LN:1606

@SQ SN:chrUn_JTFH01000487v1_decoy LN:1605

@SQ SN:chrUn_JTFH01000488v1_decoy LN:1605

@SQ SN:chrUn_JTFH01000489v1_decoy LN:1600

@SQ SN:chrUn_JTFH01000490v1_decoy LN:1598

@SQ SN:chrUn_JTFH01000491v1_decoy LN:1598

@SQ SN:chrUn_JTFH01000492v1_decoy LN:1597

@SQ SN:chrUn_JTFH01000493v1_decoy LN:1596

@SQ SN:chrUn_JTFH01000494v1_decoy LN:1595

@SQ SN:chrUn_JTFH01000495v1_decoy LN:1592

@SQ SN:chrUn_JTFH01000496v1_decoy LN:1589

@SQ SN:chrUn_JTFH01000497v1_decoy LN:1585

@SQ SN:chrUn_JTFH01000498v1_decoy LN:1579

@SQ SN:chrUn_JTFH01000499v1_decoy LN:1578

@SQ SN:chrUn_JTFH01000500v1_decoy LN:1577

@SQ SN:chrUn_JTFH01000501v1_decoy LN:1577

@SQ SN:chrUn_JTFH01000502v1_decoy LN:1577

@SQ SN:chrUn_JTFH01000503v1_decoy LN:1576

@SQ SN:chrUn_JTFH01000504v1_decoy LN:1575

@SQ SN:chrUn_JTFH01000505v1_decoy LN:1574

@SQ SN:chrUn_JTFH01000506v1_decoy LN:1572

@SQ SN:chrUn_JTFH01000507v1_decoy LN:1571

@SQ SN:chrUn_JTFH01000508v1_decoy LN:1563

@SQ SN:chrUn_JTFH01000509v1_decoy LN:1561

@SQ SN:chrUn_JTFH01000510v1_decoy LN:1561

@SQ SN:chrUn_JTFH01000511v1_decoy LN:1560

@SQ SN:chrUn_JTFH01000512v1_decoy LN:1560

@SQ SN:chrUn_JTFH01000513v1_decoy LN:1554

@SQ SN:chrUn_JTFH01000514v1_decoy LN:1552

@SQ SN:chrUn_JTFH01000515v1_decoy LN:1548

@SQ SN:chrUn_JTFH01000516v1_decoy LN:1546

@SQ SN:chrUn_JTFH01000517v1_decoy LN:1541

@SQ SN:chrUn_JTFH01000518v1_decoy LN:1536

@SQ SN:chrUn_JTFH01000519v1_decoy LN:1533

@SQ SN:chrUn_JTFH01000520v1_decoy LN:1532

@SQ SN:chrUn_JTFH01000521v1_decoy LN:1532

@SQ SN:chrUn_JTFH01000522v1_decoy LN:1530

@SQ SN:chrUn_JTFH01000523v1_decoy LN:1527

@SQ SN:chrUn_JTFH01000524v1_decoy LN:1526

@SQ SN:chrUn_JTFH01000525v1_decoy LN:1524

@SQ SN:chrUn_JTFH01000526v1_decoy LN:1523

@SQ SN:chrUn_JTFH01000527v1_decoy LN:1523

@SQ SN:chrUn_JTFH01000528v1_decoy LN:1522

@SQ SN:chrUn_JTFH01000529v1_decoy LN:1522

@SQ SN:chrUn_JTFH01000530v1_decoy LN:1519

@SQ SN:chrUn_JTFH01000531v1_decoy LN:1513

@SQ SN:chrUn_JTFH01000532v1_decoy LN:1508

@SQ SN:chrUn_JTFH01000533v1_decoy LN:1508

@SQ SN:chrUn_JTFH01000534v1_decoy LN:1505

@SQ SN:chrUn_JTFH01000535v1_decoy LN:1503

@SQ SN:chrUn_JTFH01000536v1_decoy LN:1496

@SQ SN:chrUn_JTFH01000537v1_decoy LN:1491

@SQ SN:chrUn_JTFH01000538v1_decoy LN:1490

@SQ SN:chrUn_JTFH01000539v1_decoy LN:1490

@SQ SN:chrUn_JTFH01000540v1_decoy LN:1487

@SQ SN:chrUn_JTFH01000541v1_decoy LN:1486

@SQ SN:chrUn_JTFH01000542v1_decoy LN:1485

@SQ SN:chrUn_JTFH01000543v1_decoy LN:1484

@SQ SN:chrUn_JTFH01000544v1_decoy LN:1483

@SQ SN:chrUn_JTFH01000545v1_decoy LN:1479

@SQ SN:chrUn_JTFH01000546v1_decoy LN:1479

@SQ SN:chrUn_JTFH01000547v1_decoy LN:1476

@SQ SN:chrUn_JTFH01000548v1_decoy LN:1475

@SQ SN:chrUn_JTFH01000549v1_decoy LN:1472

@SQ SN:chrUn_JTFH01000550v1_decoy LN:1469

@SQ SN:chrUn_JTFH01000551v1_decoy LN:1468

@SQ SN:chrUn_JTFH01000552v1_decoy LN:1467

@SQ SN:chrUn_JTFH01000553v1_decoy LN:1465

@SQ SN:chrUn_JTFH01000554v1_decoy LN:1464

@SQ SN:chrUn_JTFH01000555v1_decoy LN:1463

@SQ SN:chrUn_JTFH01000556v1_decoy LN:1463

@SQ SN:chrUn_JTFH01000557v1_decoy LN:1459

@SQ SN:chrUn_JTFH01000558v1_decoy LN:1459

@SQ SN:chrUn_JTFH01000559v1_decoy LN:1458

@SQ SN:chrUn_JTFH01000560v1_decoy LN:1458

@SQ SN:chrUn_JTFH01000561v1_decoy LN:1454

@SQ SN:chrUn_JTFH01000562v1_decoy LN:1449

@SQ SN:chrUn_JTFH01000563v1_decoy LN:1449

@SQ SN:chrUn_JTFH01000564v1_decoy LN:1448

@SQ SN:chrUn_JTFH01000565v1_decoy LN:1446

@SQ SN:chrUn_JTFH01000566v1_decoy LN:1442

@SQ SN:chrUn_JTFH01000567v1_decoy LN:1441

@SQ SN:chrUn_JTFH01000568v1_decoy LN:1440

@SQ SN:chrUn_JTFH01000569v1_decoy LN:1439

@SQ SN:chrUn_JTFH01000570v1_decoy LN:1437

@SQ SN:chrUn_JTFH01000571v1_decoy LN:1436

@SQ SN:chrUn_JTFH01000572v1_decoy LN:1429

@SQ SN:chrUn_JTFH01000573v1_decoy LN:1429

@SQ SN:chrUn_JTFH01000574v1_decoy LN:1427

@SQ SN:chrUn_JTFH01000575v1_decoy LN:1426

@SQ SN:chrUn_JTFH01000576v1_decoy LN:1425

@SQ SN:chrUn_JTFH01000577v1_decoy LN:1424

@SQ SN:chrUn_JTFH01000578v1_decoy LN:1424

@SQ SN:chrUn_JTFH01000579v1_decoy LN:1423

@SQ SN:chrUn_JTFH01000580v1_decoy LN:1423

@SQ SN:chrUn_JTFH01000581v1_decoy LN:1423

@SQ SN:chrUn_JTFH01000582v1_decoy LN:1414

@SQ SN:chrUn_JTFH01000583v1_decoy LN:1414

@SQ SN:chrUn_JTFH01000584v1_decoy LN:1413

@SQ SN:chrUn_JTFH01000585v1_decoy LN:1413

@SQ SN:chrUn_JTFH01000586v1_decoy LN:1410

@SQ SN:chrUn_JTFH01000587v1_decoy LN:1409

@SQ SN:chrUn_JTFH01000588v1_decoy LN:1409

@SQ SN:chrUn_JTFH01000589v1_decoy LN:1406

@SQ SN:chrUn_JTFH01000590v1_decoy LN:1405

@SQ SN:chrUn_JTFH01000591v1_decoy LN:1405

@SQ SN:chrUn_JTFH01000592v1_decoy LN:1404

@SQ SN:chrUn_JTFH01000593v1_decoy LN:1404

@SQ SN:chrUn_JTFH01000594v1_decoy LN:1402

@SQ SN:chrUn_JTFH01000595v1_decoy LN:1402

@SQ SN:chrUn_JTFH01000596v1_decoy LN:1402

@SQ SN:chrUn_JTFH01000597v1_decoy LN:1402

@SQ SN:chrUn_JTFH01000598v1_decoy LN:1400

@SQ SN:chrUn_JTFH01000599v1_decoy LN:1398

@SQ SN:chrUn_JTFH01000600v1_decoy LN:1396

@SQ SN:chrUn_JTFH01000601v1_decoy LN:1395

@SQ SN:chrUn_JTFH01000602v1_decoy LN:1394

@SQ SN:chrUn_JTFH01000603v1_decoy LN:1393

@SQ SN:chrUn_JTFH01000604v1_decoy LN:1391

@SQ SN:chrUn_JTFH01000605v1_decoy LN:1389

@SQ SN:chrUn_JTFH01000606v1_decoy LN:1389

@SQ SN:chrUn_JTFH01000607v1_decoy LN:1388

@SQ SN:chrUn_JTFH01000608v1_decoy LN:1387

@SQ SN:chrUn_JTFH01000609v1_decoy LN:1384

@SQ SN:chrUn_JTFH01000610v1_decoy LN:1381

@SQ SN:chrUn_JTFH01000611v1_decoy LN:1381

@SQ SN:chrUn_JTFH01000612v1_decoy LN:1379

@SQ SN:chrUn_JTFH01000613v1_decoy LN:1377

@SQ SN:chrUn_JTFH01000614v1_decoy LN:1376

@SQ SN:chrUn_JTFH01000615v1_decoy LN:1376

@SQ SN:chrUn_JTFH01000616v1_decoy LN:1375

@SQ SN:chrUn_JTFH01000617v1_decoy LN:1374

@SQ SN:chrUn_JTFH01000618v1_decoy LN:1372

@SQ SN:chrUn_JTFH01000619v1_decoy LN:1371

@SQ SN:chrUn_JTFH01000620v1_decoy LN:1370

@SQ SN:chrUn_JTFH01000621v1_decoy LN:1370

@SQ SN:chrUn_JTFH01000622v1_decoy LN:1366

@SQ SN:chrUn_JTFH01000623v1_decoy LN:1363

@SQ SN:chrUn_JTFH01000624v1_decoy LN:1360

@SQ SN:chrUn_JTFH01000625v1_decoy LN:1356

@SQ SN:chrUn_JTFH01000626v1_decoy LN:1355

@SQ SN:chrUn_JTFH01000627v1_decoy LN:1355

@SQ SN:chrUn_JTFH01000628v1_decoy LN:1352

@SQ SN:chrUn_JTFH01000629v1_decoy LN:1345

@SQ SN:chrUn_JTFH01000630v1_decoy LN:1344

@SQ SN:chrUn_JTFH01000631v1_decoy LN:1344

@SQ SN:chrUn_JTFH01000632v1_decoy LN:1342

@SQ SN:chrUn_JTFH01000633v1_decoy LN:1342

@SQ SN:chrUn_JTFH01000634v1_decoy LN:1336

@SQ SN:chrUn_JTFH01000635v1_decoy LN:1334

@SQ SN:chrUn_JTFH01000636v1_decoy LN:1334

@SQ SN:chrUn_JTFH01000637v1_decoy LN:1333

@SQ SN:chrUn_JTFH01000638v1_decoy LN:1332

@SQ SN:chrUn_JTFH01000639v1_decoy LN:1328

@SQ SN:chrUn_JTFH01000640v1_decoy LN:1328

@SQ SN:chrUn_JTFH01000641v1_decoy LN:1328

@SQ SN:chrUn_JTFH01000642v1_decoy LN:1327

@SQ SN:chrUn_JTFH01000643v1_decoy LN:1325

@SQ SN:chrUn_JTFH01000644v1_decoy LN:1322

@SQ SN:chrUn_JTFH01000645v1_decoy LN:1320

@SQ SN:chrUn_JTFH01000646v1_decoy LN:1319

@SQ SN:chrUn_JTFH01000647v1_decoy LN:1318

@SQ SN:chrUn_JTFH01000648v1_decoy LN:1315

@SQ SN:chrUn_JTFH01000649v1_decoy LN:1314

@SQ SN:chrUn_JTFH01000650v1_decoy LN:1313

@SQ SN:chrUn_JTFH01000651v1_decoy LN:1313

@SQ SN:chrUn_JTFH01000652v1_decoy LN:1312

@SQ SN:chrUn_JTFH01000653v1_decoy LN:1310

@SQ SN:chrUn_JTFH01000654v1_decoy LN:1309

@SQ SN:chrUn_JTFH01000655v1_decoy LN:1309

@SQ SN:chrUn_JTFH01000656v1_decoy LN:1307

@SQ SN:chrUn_JTFH01000657v1_decoy LN:1307

@SQ SN:chrUn_JTFH01000658v1_decoy LN:1305

@SQ SN:chrUn_JTFH01000659v1_decoy LN:1304

@SQ SN:chrUn_JTFH01000660v1_decoy LN:1303

@SQ SN:chrUn_JTFH01000661v1_decoy LN:1302

@SQ SN:chrUn_JTFH01000662v1_decoy LN:1302

@SQ SN:chrUn_JTFH01000663v1_decoy LN:1301

@SQ SN:chrUn_JTFH01000664v1_decoy LN:1301

@SQ SN:chrUn_JTFH01000665v1_decoy LN:1300

@SQ SN:chrUn_JTFH01000666v1_decoy LN:1299

@SQ SN:chrUn_JTFH01000667v1_decoy LN:1297

@SQ SN:chrUn_JTFH01000668v1_decoy LN:1295

@SQ SN:chrUn_JTFH01000669v1_decoy LN:1294

@SQ SN:chrUn_JTFH01000670v1_decoy LN:1293

@SQ SN:chrUn_JTFH01000671v1_decoy LN:1291

@SQ SN:chrUn_JTFH01000672v1_decoy LN:1291

@SQ SN:chrUn_JTFH01000673v1_decoy LN:1289

@SQ SN:chrUn_JTFH01000674v1_decoy LN:1288

@SQ SN:chrUn_JTFH01000675v1_decoy LN:1288

@SQ SN:chrUn_JTFH01000676v1_decoy LN:1287

@SQ SN:chrUn_JTFH01000677v1_decoy LN:1287

@SQ SN:chrUn_JTFH01000678v1_decoy LN:1287

@SQ SN:chrUn_JTFH01000679v1_decoy LN:1286

@SQ SN:chrUn_JTFH01000680v1_decoy LN:1283

@SQ SN:chrUn_JTFH01000681v1_decoy LN:1281

@SQ SN:chrUn_JTFH01000682v1_decoy LN:1277

@SQ SN:chrUn_JTFH01000683v1_decoy LN:1274

@SQ SN:chrUn_JTFH01000684v1_decoy LN:1270

@SQ SN:chrUn_JTFH01000685v1_decoy LN:1267

@SQ SN:chrUn_JTFH01000686v1_decoy LN:1266

@SQ SN:chrUn_JTFH01000687v1_decoy LN:1260

@SQ SN:chrUn_JTFH01000688v1_decoy LN:1259

@SQ SN:chrUn_JTFH01000689v1_decoy LN:1258

@SQ SN:chrUn_JTFH01000690v1_decoy LN:1258

@SQ SN:chrUn_JTFH01000691v1_decoy LN:1258

@SQ SN:chrUn_JTFH01000692v1_decoy LN:1256

@SQ SN:chrUn_JTFH01000693v1_decoy LN:1255

@SQ SN:chrUn_JTFH01000694v1_decoy LN:1254

@SQ SN:chrUn_JTFH01000695v1_decoy LN:1254

@SQ SN:chrUn_JTFH01000696v1_decoy LN:1253

@SQ SN:chrUn_JTFH01000697v1_decoy LN:1250

@SQ SN:chrUn_JTFH01000698v1_decoy LN:1249

@SQ SN:chrUn_JTFH01000699v1_decoy LN:1248

@SQ SN:chrUn_JTFH01000700v1_decoy LN:1248

@SQ SN:chrUn_JTFH01000701v1_decoy LN:1247

@SQ SN:chrUn_JTFH01000702v1_decoy LN:1242

@SQ SN:chrUn_JTFH01000703v1_decoy LN:1242

@SQ SN:chrUn_JTFH01000704v1_decoy LN:1241

@SQ SN:chrUn_JTFH01000705v1_decoy LN:1241

@SQ SN:chrUn_JTFH01000706v1_decoy LN:1241

@SQ SN:chrUn_JTFH01000707v1_decoy LN:1239

@SQ SN:chrUn_JTFH01000708v1_decoy LN:1238

@SQ SN:chrUnJTFH01000709v1

heewookl commented 5 years ago

Thanks for providing the header for the bam. I think the message may have gotten truncated because of the length. Could you also provide the output for -c flag? Thanks!

samtools view -c <initial_bam>

afadda91 commented 5 years ago

/Applications/samtools-1.9/samtools view -c /Users/afadda/Documents/MODY_family/0200172730_hs38HD_sorted.bam

779221277

You probably wanted to know if my genome contains HLA sequence. Here’s the rest of the samtools view –H

SN:HLA-A*01:01:01:01 LN:3503

@SQ SN:HLA-A*01:01:01:02N LN:3291

@SQ SN:HLA-A*01:01:38L LN:3374

@SQ SN:HLA-A*01:02 LN:3374

@SQ SN:HLA-A*01:03 LN:3503

@SQ SN:HLA-A*01:04N LN:3136

@SQ SN:HLA-A*01:09 LN:3105

@SQ SN:HLA-A*01:11N LN:3374

@SQ SN:HLA-A*01:14 LN:3095

@SQ SN:HLA-A*01:16N LN:2985

@SQ SN:HLA-A*01:20 LN:3105

@SQ SN:HLA-A*02:01:01:01 LN:3517

@SQ SN:HLA-A*02:01:01:02L LN:3287

@SQ SN:HLA-A*02:01:01:03 LN:3023

@SQ SN:HLA-A*02:01:01:04 LN:3516

@SQ SN:HLA-A*02:02:01 LN:2917

@SQ SN:HLA-A*02:03:01 LN:3517

@SQ SN:HLA-A*02:03:03 LN:3148

@SQ SN:HLA-A*02:05:01 LN:3517

@SQ SN:HLA-A*02:06:01 LN:3517

@SQ SN:HLA-A*02:07:01 LN:3517

@SQ SN:HLA-A*02:10 LN:3517

@SQ SN:HLA-A*02:251 LN:3517

@SQ SN:HLA-A*02:259 LN:2978

@SQ SN:HLA-A*02:264 LN:3002

@SQ SN:HLA-A*02:265 LN:3148

@SQ SN:HLA-A*02:266 LN:3084

@SQ SN:HLA-A*02:269 LN:3101

@SQ SN:HLA-A*02:279 LN:3103

@SQ SN:HLA-A*02:32N LN:3517

@SQ SN:HLA-A*02:376 LN:3104

@SQ SN:HLA-A*02:43N LN:3218

@SQ SN:HLA-A*02:455 LN:3118

@SQ SN:HLA-A*02:48 LN:3517

@SQ SN:HLA-A*02:51 LN:3109

@SQ SN:HLA-A*02:533 LN:3217

@SQ SN:HLA-A*02:53N LN:3305

@SQ SN:HLA-A*02:57 LN:3054

@SQ SN:HLA-A*02:60:01 LN:3112

@SQ SN:HLA-A*02:65 LN:3387

@SQ SN:HLA-A*02:68 LN:3109

@SQ SN:HLA-A*02:77 LN:3371

@SQ SN:HLA-A*02:81 LN:3309

@SQ SN:HLA-A*02:89 LN:3371

@SQ SN:HLA-A*02:95 LN:3388

@SQ SN:HLA-A*03:01:01:01 LN:3502

@SQ SN:HLA-A*03:01:01:02N LN:3373

@SQ SN:HLA-A*03:01:01:03 LN:3094

@SQ SN:HLA-A*03:02:01 LN:3502

@SQ SN:HLA-A*03:11N LN:3404

@SQ SN:HLA-A*03:21N LN:3095

@SQ SN:HLA-A*03:36N LN:3142

@SQ SN:HLA-A*11:01:01 LN:3503

@SQ SN:HLA-A*11:01:18 LN:3503

@SQ SN:HLA-A*11:02:01 LN:3503

@SQ SN:HLA-A*11:05 LN:3373

@SQ SN:HLA-A*11:110 LN:2903

@SQ SN:HLA-A*11:25 LN:3073

@SQ SN:HLA-A*11:50Q LN:3362

@SQ SN:HLA-A*11:60 LN:3241

@SQ SN:HLA-A*11:69N LN:3500

@SQ SN:HLA-A*11:74 LN:3227

@SQ SN:HLA-A*11:75 LN:3184

@SQ SN:HLA-A*11:77 LN:3233

@SQ SN:HLA-A*23:01:01 LN:3502

@SQ SN:HLA-A*23:09 LN:3104

@SQ SN:HLA-A*23:38N LN:3020

@SQ SN:HLA-A*24:02:01:01 LN:3502

@SQ SN:HLA-A*24:02:01:02L LN:3502

@SQ SN:HLA-A*24:02:01:03 LN:3075

@SQ SN:HLA-A*24:02:03Q LN:3247

@SQ SN:HLA-A*24:02:10 LN:3356

@SQ SN:HLA-A*24:03:01 LN:3502

@SQ SN:HLA-A*24:07:01 LN:3502

@SQ SN:HLA-A*24:08 LN:3502

@SQ SN:HLA-A*24:09N LN:3502

@SQ SN:HLA-A*24:10:01 LN:3502

@SQ SN:HLA-A*24:11N LN:3503

@SQ SN:HLA-A*24:152 LN:3176

@SQ SN:HLA-A*24:20 LN:3502

@SQ SN:HLA-A*24:215 LN:3116

@SQ SN:HLA-A*24:61 LN:3043

@SQ SN:HLA-A*24:86N LN:3415

@SQ SN:HLA-A*25:01:01 LN:2917

@SQ SN:HLA-A*26:01:01 LN:3517

@SQ SN:HLA-A*26:11N LN:3091

@SQ SN:HLA-A*26:15 LN:3217

@SQ SN:HLA-A*26:50 LN:3141

@SQ SN:HLA-A*29:01:01:01 LN:3518

@SQ SN:HLA-A*29:01:01:02N LN:3303

@SQ SN:HLA-A*29:02:01:01 LN:3518

@SQ SN:HLA-A*29:02:01:02 LN:3518

@SQ SN:HLA-A*29:46 LN:3310

@SQ SN:HLA-A*30:01:01 LN:3503

@SQ SN:HLA-A*30:02:01:01 LN:2903

@SQ SN:HLA-A*30:02:01:02 LN:3374

@SQ SN:HLA-A*30:04:01 LN:3503

@SQ SN:HLA-A*30:89 LN:2903

@SQ SN:HLA-A*31:01:02 LN:3518

@SQ SN:HLA-A*31:01:23 LN:2918

@SQ SN:HLA-A*31:04 LN:2918

@SQ SN:HLA-A*31:14N LN:3090

@SQ SN:HLA-A*31:46 LN:3075

@SQ SN:HLA-A*32:01:01 LN:3518

@SQ SN:HLA-A*32:06 LN:3389

@SQ SN:HLA-A*33:01:01 LN:3518

@SQ SN:HLA-A*33:03:01 LN:3518

@SQ SN:HLA-A*33:07 LN:3389

@SQ SN:HLA-A*34:01:01 LN:3517

@SQ SN:HLA-A*34:02:01 LN:3096

@SQ SN:HLA-A*36:01 LN:2903

@SQ SN:HLA-A*43:01 LN:3388

@SQ SN:HLA-A*66:01:01 LN:3517

@SQ SN:HLA-A*66:17 LN:3075

@SQ SN:HLA-A*68:01:01:01 LN:2930

@SQ SN:HLA-A*68:01:01:02 LN:3517

@SQ SN:HLA-A*68:01:02:01 LN:3517

@SQ SN:HLA-A*68:01:02:02 LN:3388

@SQ SN:HLA-A*68:02:01:01 LN:3517

@SQ SN:HLA-A*68:02:01:02 LN:3506

@SQ SN:HLA-A*68:02:01:03 LN:2909

@SQ SN:HLA-A*68:02:02 LN:2916

@SQ SN:HLA-A*68:03:01 LN:2917

@SQ SN:HLA-A*68:08:01 LN:3120

@SQ SN:HLA-A*68:113 LN:3070

@SQ SN:HLA-A*68:17 LN:3134

@SQ SN:HLA-A*68:18N LN:3237

@SQ SN:HLA-A*68:22 LN:3119

@SQ SN:HLA-A*68:71 LN:3198

@SQ SN:HLA-A*69:01 LN:2917

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@SQ SN:HLA-DRB1*12:17 LN:11260

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@SQ SN:HLA-DRB1*15:01:01:01 LN:11080

@SQ SN:HLA-DRB1*15:01:01:02 LN:11571

@SQ SN:HLA-DRB1*15:01:01:03 LN:11056

@SQ SN:HLA-DRB1*15:01:01:04 LN:11056

@SQ SN:HLA-DRB1*15:02:01 LN:10313

@SQ SN:HLA-DRB1*15:03:01:01 LN:11567

@SQ SN:HLA-DRB1*15:03:01:02 LN:11569

@SQ SN:HLA-DRB1*16:02:01 LN:11005

@RG

From: Heewook Lee notifications@github.com<mailto:notifications@github.com> Reply-To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com> Date: Wednesday, November 28, 2018 at 6:55 PM To: Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org>, Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

Thanks for providing the header for the bam. I think the message may have gotten truncated because of the length. Could you also provide the output for -c flag? Thanks!

samtools view -c

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afadda91 commented 5 years ago

Dear,

Did you figure out the issue of not having more genes typed with 30x coverage? We’re interested in running your tool on thousands of such data, and would like to know if it fits the job.

Thanks,

Abeer

From: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org> Date: Thursday, November 29, 2018 at 3:35 PM To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com>, Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

/Applications/samtools-1.9/samtools view -c /Users/afadda/Documents/MODY_family/0200172730_hs38HD_sorted.bam

779221277

You probably wanted to know if my genome contains HLA sequence. Here’s the rest of the samtools view –H

SN:HLA-A*01:01:01:01 LN:3503

@SQSN:HLA-A*01:01:01:02NLN:3291

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@SQSN:HLA-A*03:01:01:02NLN:3373

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@SQSN:HLA-B*27:06 LN:3325

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@SQSN:HLA-B*35:01:22 LN:2806

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@SQSN:HLA-B*35:14:02 LN:3327

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@SQSN:HLA-C*01:02:29 LN:3349

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@SQSN:HLA-C*02:11 LN:3320

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@SQSN:HLA-C*02:86 LN:3347

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@SQSN:HLA-C*04:06 LN:3349

@SQSN:HLA-C*04:09N LN:2991

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@SQSN:HLA-C*04:161 LN:3237

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@SQSN:HLA-C*05:08 LN:3059

@SQSN:HLA-C*05:09:01 LN:3322

@SQSN:HLA-C*05:93 LN:2946

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@SQSN:HLA-C*06:02:01:02 LN:3349

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@SQSN:HLA-C*06:23 LN:3349

@SQSN:HLA-C*06:24 LN:3349

@SQSN:HLA-C*06:46N LN:2987

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@SQSN:HLA-C*07:01:01:02 LN:3093

@SQSN:HLA-C*07:01:02 LN:3352

@SQSN:HLA-C*07:01:19 LN:3354

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@SQSN:HLA-DQB1*06:09:01 LN:7102

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@RG

From: Heewook Lee notifications@github.com<mailto:notifications@github.com> Reply-To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com> Date: Wednesday, November 28, 2018 at 6:55 PM To: Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org>, Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

Thanks for providing the header for the bam. I think the message may have gotten truncated because of the length. Could you also provide the output for -c flag? Thanks!

samtools view -c

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afadda91 commented 5 years ago

Hi,

I would appreciate if you took the time to look into this. It’s very important!

Thanks you

[cid:43784F07-9918-4382-A6A4-300143ED58AD]

Abeer Fadda, PhD Staff Scientist, Bioinformatics, Systems Biology Division t. +974 4003 7629 Office C6 313 OPC PO BOX 26999 Doha, Qatar sidra.orghttp://www.sidra.org/

heewookl commented 5 years ago

Hi,

I will try to take a look at it next week. Would it be possible for you to share one of the bam files that you had trouble with? This will help me when I try to figure why some genes were not getting assembled. Thank you.

afadda91 commented 5 years ago

Sure. How do you suggest I share it?

Abeer

From: Heewook Lee notifications@github.com<mailto:notifications@github.com> Reply-To: Kingsford-Group/kourami reply@reply.github.com<mailto:reply@reply.github.com> Date: Saturday, March 2, 2019 at 1:11 AM To: Kingsford-Group/kourami kourami@noreply.github.com<mailto:kourami@noreply.github.com> Cc: Abeer Fadda afadda@sidra.org<mailto:afadda@sidra.org>, Author author@noreply.github.com<mailto:author@noreply.github.com> Subject: Re: [Kingsford-Group/kourami] typing additional loci (#20)

Hi,

I will try to take a look at it next week. Would it be possible for you to share one of the bam files that you had trouble with? This will help me when I try to figure why some genes were not getting assembled. Thank you.

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freshfischer commented 4 years ago

hi, when i use the -a option it only types A,B,C,DQA1,DRB1. if i specify -a MICA for instance, i get the message "Input bam : A DOES NOT exist. Please check the bam exists." I know the MICA reads are present in the KouramiPanel.bam. So what is the problem. Also, is there an explanation to the numbers that come in the results file ?

thanks

-a option seems to caculate and output all the loci that aligned to hg38 reference and IMGT/IPD HLA database. code as follow: $kourami/scripts/alignAndExtract_hs38DH_NoAlt.sh -d $kourami/custom_db/3.40.0/ -r $kourami/resource $sample $inbam

java -jar $kourami/build/Kourami.jar -d $kourami/custom_db/3.40.0/ -a -o $sample ${sample}_on_KouramiPanel.bam

prmac commented 3 years ago

The bam does not exist error has nothing to do with -a flag. Could you please provide the entire command line you used to run Kourami?

-a flag does not take any value. -a flag just triggers Kourami to type pre-defined additional loci: DOA DMA DMB DPA1 DPB1 DRA DRB3 DRB5 F H J L

MICA locus is currently not supported.

Is MICA supported now?

Kingsford-Group / kourami

typing additional loci #20