Hello!
I get this error while running Squid. The chimeric sam file generated through STAR run is empty and it fails when run as a bam file with Squid. Please help me to sort this error out. Is it because the chimeric reads are not detected for the datasets I used?
/home/software/STAR-2.7.9a/bin/Linux_x86_64/STAR --runThreadN 55 --genomeDir /home/genome_files/CTAT_Library/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --readFilesIn /home/results/read_1_trim.fastq /home/results/read_2_trim.fastq --outFileNamePrefix /home/results/star_run. --outSAMtype BAM SortedByCoordinate --outReadsUnmapped Fastx --chimSegmentMin 20 --outSAMstrandField intronMotif --chimOutType SeparateSAMold
STAR version: 2.7.9a compiled: 2021-05-04T09:43:56-0400 vega:/homedobin/data/STAR/STARcode/STAR.master/source
Sep 28 18:13:51 ..... started STAR run
Sep 28 18:13:51 ..... loading genome
Sep 28 18:13:53 ..... started mapping
Sep 28 18:14:21 ..... finished mapping
Sep 28 18:14:21 ..... started sorting BAM
Sep 28 18:14:22 ..... finished successfully
Reference name 1 --> 0
Reference name 2 --> 1
Reference name 3 --> 2
Reference name 4 --> 3
Reference name 5 --> 4
Reference name Mt --> 5
Reference name Pt --> 6
[Tue Sep 28 18:14:23 2021] Start reading bam file.
[Tue Sep 28 18:14:23 2021] Finish sorting Chimeric bam reads.
[Tue Sep 28 18:14:23 2021] Finish removing PCR duplicates.
[Tue Sep 28 18:14:23 2021] Building nodes. |bamdiscordant|=0
[Tue Sep 28 18:14:23 2021] Building nodes, finish seeding.
../scripts/no_bloom_fusion_tools.sh: line 103: 17949 Segmentation fault (core dumped) $SQUID -b $run_dir/star_run.Aligned.sortedByCoord.out.bam -c $run_dir/star_run.Chimeric.out.bam -o $run_dir/squid_out
Hello! I get this error while running Squid. The chimeric sam file generated through STAR run is empty and it fails when run as a bam file with Squid. Please help me to sort this error out. Is it because the chimeric reads are not detected for the datasets I used?