VIV-9 - MYOS617, 619, 727

KlementineJBS / USYD_PhD_ELN

This repository forms the electronic laboratory notebook (ELN)for Klementine Burrell-Sander's PhD's

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VIV-9 - MYOS617, 619, 727 #229

Open KlementineJBS opened 4 weeks ago

KlementineJBS commented 4 weeks ago

Made up 2 x flasks using 6/9 mm55 2018 plates. One didn't sonicate well - the fragments stayed on the bottom of the tube even after agitation. (Flask B)

Used flask A for this set.

KlementineJBS commented 2 weeks ago

Overview of VIV efficacy experiments:

Other	Experiment	423	617	619	726	727	731
MIC averaged	-	2	6.67	2.67	4	4	1.33
Toxicity	-	toxic	ok	ok	toxic	ok	toxic

Given results from https://github.com/KlementineJBS/USYD_PhD_ELN/issues/234 , exclude 423, 726 and 731 from future experiments. This leaves 617, 619 and 727.

KlementineJBS commented 2 weeks ago

Need 8 x 15 larvae to test the 3 compounds above.

Untouched
PBS x PBS
mm55 x PBS
mm55 x AmB old
mm55 x AmB new
mm55 x compounds (x 3)

KlementineJBS commented 1 week ago

08.10.24

Flask A yielded 455.8 mg fungus which was enough for 6 groups. New amphotericin B hasn't arrived so I did 2 sets with the old batch.

Made up 619 fresh using the new bottle of PBS.

Began infection at 1.30 pm and treatment at 4.55 pm.

Larvae were fresh - delivered on the day.

KlementineJBS commented 1 week ago

09.10.24

Second treatment administered between 3.30 and 4 pm.

KlementineJBS commented 1 week ago

NB. For PBS controls, used PBS + 5% DMSO so that it was identical in composition to the compound treatments.

KlementineJBS commented 3 days ago

VIV-9 (injected 08-10-24) raw data.pdf

KlementineJBS commented 3 days ago