VIV master page

[KlementineJBS]

Protocol

In vivo instructions.pdf

[KlementineJBS]

Attempts

Identifier	Link	Compounds tested	Deaths in untouched	Deaths in PBS*PBS group
3	#219	-	-	6
4	#226	-	3	8
6	#222	423, 619, 727, 731	1	4
7	#225	423, 617, 619, 726, 727, 731	5	7
8	#232	617, 726, 731	7	6
9	#229	617, 619, 727	ongoing	ongoing
10	#239	617, 619, 727	ongoing	ongoing
11	#233	tbd

Compounds with 3 repeats:* 731

Compounds with 2 repeats: 423, 617, 726, 727

Compounds with 1 repeat only: 619

*Assuming that 6, 7 and 8 are all valid.

Given results from #234 , exclude 423, 726 and 731 from future experiments. This leaves 617, 619 and 727.

[KlementineJBS]

Results

Results from VIV7 and VIV8 combined.

Group 1	Group 2	p value	Significance?
Untouched	PBS*PBS	0.9118	No
PBS * PBS	mm55*AmB	< 0.0001	Yes
PBS*PBS	mm55*PBS	<0.0001	Yes
mm55*AmB	mm55*PBS	0.1872	no
mm55*AmB	mm55*423	0.0003	Yes

Some thoughts:

• larvae quality is clearly an issue as PBS control doesn't differ significantly from untouched group
• 423 is the only compound that shows improved survival but it was found to be borderline toxic in #234
• still don't know why AmB fails to improve survival (should have 30-50% survival at 10 days)
• haven't included earlier results here but so far VIV-3, VIV-6 and VIV-8 have the lowest number of deaths in the PBS control

[KlementineJBS]

More results

When combining results from VIV3, VIV6 and VIV8, the untouched and PBS control groups are significantly different (0.0387) and 423 is no longer (0.3079 vs AmB line).

2-Aminothiazoles VIV3-6-8 10-10-24.zip