Closed GuillaumeHolley closed 2 years ago
Hi,
--read-error
does not make any difference for polishing.Glad to hear that it's working well! I haven't really seen many examples for ONT + Illumina assemblies being QV50+.
Hi @fenderglass,
Thank you for your input, this was very helpful. Closing now.
Hi hi,
--read-error
in combination with--nano-corr
if I am using corrected reads for the polishing? I am using the option as of now so I know Flye accepts it but it is uncertain if it makes a difference.Thank you for your work on Hapdup. I have used it recently on a 70x ONT (R9.4, Guppy 5 SUP) + 40x Illumina (2x151bp) assembly and the resulting diploid assembly had QV50 and k-mer completeness of nearly 99% according to Merqury 1.3 (trio mode).
Guillaume