First of all, thank you for making all of these cutting edge tools for single-cell data analysis. I am analysing various CyTOF data sets where I have PBMCs from four groups of volunteers that were stimulated under three different conditions. Due to the complex design, I think SAUCIE would be a good option to try.
Unfortunately, the lack of documentation really impedes my attempt to use this tool. For example, I would like to know how you actually made the plots where you compared the cellular manifolds of acute/convalescent dengue patients etc.
In addition to how to use this tool, knowing what to do afterwards would be really helpful.
First of all, thank you for making all of these cutting edge tools for single-cell data analysis. I am analysing various CyTOF data sets where I have PBMCs from four groups of volunteers that were stimulated under three different conditions. Due to the complex design, I think SAUCIE would be a good option to try.
Unfortunately, the lack of documentation really impedes my attempt to use this tool. For example, I would like to know how you actually made the plots where you compared the cellular manifolds of acute/convalescent dengue patients etc.
In addition to how to use this tool, knowing what to do afterwards would be really helpful.
Thank you for your assistance!
Best regards, Mikhael