search

LBEM-CH / focus

High Throughput Electron Microscopy Image Processing Software
http://www.focus-em.org
GNU General Public License v2.0
35 stars 14 forks source link

Deal with the thin crystal as 2d crystal? #214

Closed weifeng8322 closed 6 years ago

weifeng8322 commented 6 years ago

Dear all, I have a crystal about 100nm thick，can I use electron tomography method to collect a series of project, and then process these projects just as 2d crystal does? The cell parameter of the crystal is (P1, 100,100,100,90,90,90) Thank you for your time! Wei.

weifeng8322 commented 6 years ago

The cell parameter of the crystal is (P1, 100Å,100Å,100Å,90°,90°,90°)

rdrighetto commented 6 years ago

Hi @weifeng8322,

Interesting question. I think these crystals are too thick to be treated as 2D crystals. However it sounds to me a perfect case for collecting electron diffraction data ("MicroED"/"NanoED"). The technique is pretty much analogous to macromolecular X-ray crystallography, but using a TEM.

Here are two references on the technique:

http://scripts.iucr.org/cgi-bin/paper?S2053273315010669 http://scripts.iucr.org/cgi-bin/paper?S2053273315022500

If you are interested in further discussing these methods please contact us by e-mail.

Best wishes,

-- Ricardo Diogo Righetto

2018-03-13 16:04 GMT+01:00 weifeng8322 notifications@github.com:

The cell parameter of the crystal is (P1, 100Å,100Å,100Å,90°,90°,90°)

— You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub https://github.com/C-CINA/focus/issues/214#issuecomment-372697289, or mute the thread https://github.com/notifications/unsubscribe-auth/AHVvf0nzylhWvie3W_7m4szqiuKR3VO2ks5td9_qgaJpZM4So2JA .