LBEM-CH / focus

High Throughput Electron Microscopy Image Processing Software

http://www.focus-em.org

GNU General Public License v2.0

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Request:Bayesian polishing in Relion3.0 #231

Closed Eladbic closed 5 years ago

Eladbic commented 6 years ago

The new version of Relion have the option of Bayesian polishing. To do it one need to run the motioncor within Relion because required metadata about the motion correction is written out by the Relion-motioncor.

Can this metadata being written out?

rdrighetto commented 6 years ago

Hi,

Please send us an example of such metadata (I understand it is a new .star file) and we will look into implementing it. Another possibility would be to run Relion's MotionCor2 implementation from within FOCUS.

Eladbic commented 6 years ago

Hi, Thanks for your help. This is an example for the new .star file from Relion motioncor2 job:

data_

loop_ _rlnMicrographName #1 _rlnMicrographMetadata #2 _rlnAccumMotionTotal #3 _rlnAccumMotionEarly #4 _rlnAccumMotionLate #5 MotionCorr/job061/xenoA_2169.mrc MotionCorr/job061/xenoA_2169.star 15.637464 1.189596 14.447868 MotionCorr/job061/xenoA_2168.mrc MotionCorr/job061/xenoA_2168.star 17.497252 0.852047 16.645206

Best,

rdrighetto commented 5 years ago

FOCUS now exports the metadata from MotionCor2 in the format expected for Bayesian polishing in RELION-3. Commit 77edb1d14178ab44b594d1fdbbaf09e83fe17a25

Eladbic commented 5 years ago

Thanks for your help.