Good day!
As the default settings from 'dada2_chimera_taxa.R' workflows. I get some output files including seqtab and tax table.
I find nearly all of my unique variants were assigned to unclassified taxa, even in phylum or class level.
However, when I use the 'assign_taxonomy.py', I was getting normal results.
Good day! As the default settings from 'dada2_chimera_taxa.R' workflows. I get some output files including seqtab and tax table. I find nearly all of my unique variants were assigned to unclassified taxa, even in phylum or class level. However, when I use the 'assign_taxonomy.py', I was getting normal results.