I have been using seqfold to design oligo constructs, but have run into issues when I use degenerate bases (like in unique molecular identifiers, UMIs). Would there be a straightforward way to allow for degenerate bases? A minimal version of this might be to ignore their interactions, but include them in the backbone? Thanks!
I have been using seqfold to design oligo constructs, but have run into issues when I use degenerate bases (like in unique molecular identifiers, UMIs). Would there be a straightforward way to allow for degenerate bases? A minimal version of this might be to ignore their interactions, but include them in the backbone? Thanks!