NinaKlos
commented
2 years ago 2. Crude cell extract Messung mit Thermo Scientifc HPLC
Initial conditions: 40 mM (R)-3-OH-PAC, 15 mg/mL crude cell extract, 250 mM IPA, 1 mM PLP
pH controlled every hour to 7.5 Vacuum: 750 mbar after 4h adding 40 mM (R)-3-OH-PAC, 15 mg/mL crude cell extract, 250 mM IPA
Result:
mit Konzentrationsanpassung nach Zugabe um den Faktor 1.2:
1. Whole cells Messung mit Thermo Scientific HPLC
Inital reaction condition: cell concentration: 20 mg/mL, (R)-3-OH-PAC: 80 mM, IPA: 500 mM, PLP: 1 mM
Vacuum: 750 mbar
After 4.5 h add: 10 mg/mL lyophilized whole cells, 100 mM IPA (1.69 mL)
pH-value controlled to pH 7.5 after 5h and 8.5h
Result:
zweiter Peak nach Metaraminol sichtbar, Benzoat messbar