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LernerLab / GuPPy

Guided Photometry Analysis in Python, a free and open-source FP analysis tool.
GNU General Public License v3.0
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Wanting to not use isosbestic channel that's in my data #57

Closed h1408 closed 9 months ago

h1408 commented 10 months ago

Hi, I am trying to use guppy to get control channels as if there were no isosbestic channel (even though there is data from a 405 channel from my TDT recording), and I do set that parameter to 'False' when saving input parameters, however I do not see output in the terminal that it is creating a control channel. Rather, I see that it says that data is fetched and saved for the 405 channel from the Tev/Tsq file. Is there anything else I need to do in order to make sure I can get a simulated control channel from guppy and not use the 405 from my TDT data? Thank you in advance for your help!

venus-sherathiya commented 10 months ago

Try to complete Extract timestamps and its correction step and see if it gets completed without any errors

venus-sherathiya commented 10 months ago

Also, please make sure not to use 405 storename while saving storeslist file in Storenames GUI

h1408 commented 10 months ago

Hi thanks so much! I did that and I think it is fixed now, but what is the difference between 'control_BNST.hdf5' output and the 'cntrl0.hdf5' output? Which of those is used as the F for the dff calculation?

venus-sherathiya commented 10 months ago

To answer your question, can you please delete the output folder and run the analysis again. After that, please send me the screenshot of the output folder and storeslist.csv file ?

h1408 commented 10 months ago

Hi, thanks! Yes I ran it on a new recording. Here are the screenshots: Screenshot 2023-10-26 at 2 08 37 PM

Screenshot 2023-10-26 at 2 08 51 PM

venus-sherathiya commented 10 months ago

control_BNST is used for dff calculation

h1408 commented 10 months ago

Okay and is 465A or the signal_BNST traces used as the signal in for dff? Also is cntrl0 is used for anything? Because when I plot it seems to follow the 465A trace (see below). I appreciate your help with this!

Screenshot 2023-10-26 at 2 14 51 PM

venus-sherathiya commented 10 months ago

cntrl0 is similar to 465A. cntrl0 is used to compute control_BNST. control_BNST and signal_BNST are used for dff calculation.

venus-sherathiya commented 9 months ago

Hello, Should I close this issue ?

h1408 commented 9 months ago

Hi, yes thank you so much for your help!

venus-sherathiya commented 9 months ago

issue resolved !!