Open danielkaifeng opened 1 year ago
Hi Daniel, could you send me the “Reference” folder and also the sam file in Elution-3 sample (under Analysis/) for me to debug?
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @.> @.
发件人: daniel_deng @.*** 发送时间: 2023年2月9日 10:57 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: Subscribed 主题: [EXTERNAL][LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
I got error when running java -jar DEL_AnalysisToolkit_NAR.jar project -proj exp_2023020818:
[2023/02/08 17:48:07] ----1D1D-------- [102/372] [2023/02/08 17:48:38] ----1D1D----1R-- [2023/02/08 17:48:38] ----1D1D----1D-- [2023/02/08 17:49:00] ----2R1D----1D-- [2023/02/08 17:49:00] ----2D1D----1D-- [2023/02/08 17:49:11] ----2D1D----1D1R [2023/02/08 17:49:11] ----2D1D----1D1D [2023/02/08 17:49:19] ----3R1D----1D1D Fail to run molcal for Elution-3
cat step3.stderr
Exception in thread "main" java.lang.NullPointerException at DEL_AnalysisToolkit.DEL_Reads2Compounds.runMolCal(DEL_Reads2Compounds.java:384) at DEL_AnalysisToolkit.DEL_AnalysisToolkit.main(DEL_AnalysisToolkit.java:65)
My environment:
Ubuntu 18.04.6 LTS R version 4.2.0 (2022-04-22) openjdk version "1.8.0_312"
library('ggplot2') library('reshape2') library('gridExtra')
Library Preparation
Libraries were prepared using OpenDeL: java -jar DEL_AnalysisToolkit_NAR.jar convopendel -f lib_path.spapb -outdir lib library index files were placed in samples directory:
==> lib/librarylist.expID.txt <== 0 0 0 0 0 0 0 0 0 0
==> lib/librarylist.txt <== lib/new_similarity_NReagent_ReviR-OpenDEL0001.txt lib/new_similarity_NReagent_ReviR-OpenDEL0002.txt lib/new_similarity_NReagent_ReviR-OpenDEL0003.txt lib/new_similarity_NReagent_ReviR-OpenDEL0004.txt lib/new_similarity_NReagent_ReviR-OpenDEL0005.txt lib/new_similarity_NReagent_ReviR-OpenDEL0006.txt lib/new_similarity_NReagent_ReviR-OpenDEL0007.txt lib/new_similarity_NReagent_ReviR-OpenDEL0008.txt lib/new_similarity_NReagent_ReviR-OpenDEL0009.txt lib/new_similarity_NReagent_ReviR-OpenDEL0010.txt
Many thanks for your help!
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAV6ZR4Y7BR27VKFC2TWWRMGLANCNFSM6AAAAAAUWAFVGA . You are receiving this because you are subscribed to this thread.Message ID: @.***>
Yes, here is the files. Thanks a lot for your attention.
--
Daniel Deng Cell: 15521321947 E-mail: @.***
At 2023-02-09 11:07:36, "LiYouBioinfo" @.***> wrote:
Hi Daniel, could you send me the “Reference” folder and also the sam file in Elution-3 sample (under Analysis/) for me to debug?
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @.> @.
发件人: daniel_deng @.*** 发送时间: 2023年2月9日 10:57 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: Subscribed 主题: [EXTERNAL][LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
I got error when running java -jar DEL_AnalysisToolkit_NAR.jar project -proj exp_2023020818:
[2023/02/08 17:48:07] ----1D1D-------- [102/372] [2023/02/08 17:48:38] ----1D1D----1R-- [2023/02/08 17:48:38] ----1D1D----1D-- [2023/02/08 17:49:00] ----2R1D----1D-- [2023/02/08 17:49:00] ----2D1D----1D-- [2023/02/08 17:49:11] ----2D1D----1D1R [2023/02/08 17:49:11] ----2D1D----1D1D [2023/02/08 17:49:19] ----3R1D----1D1D Fail to run molcal for Elution-3
cat step3.stderr
Exception in thread "main" java.lang.NullPointerException at DEL_AnalysisToolkit.DEL_Reads2Compounds.runMolCal(DEL_Reads2Compounds.java:384) at DEL_AnalysisToolkit.DEL_AnalysisToolkit.main(DEL_AnalysisToolkit.java:65)
My environment:
Ubuntu 18.04.6 LTS R version 4.2.0 (2022-04-22) openjdk version "1.8.0_312"
library('ggplot2') library('reshape2') library('gridExtra')
Library Preparation
Libraries were prepared using OpenDeL: java -jar DEL_AnalysisToolkit_NAR.jar convopendel -f lib_path.spapb -outdir lib library index files were placed in samples directory:
==> lib/librarylist.expID.txt <== 0 0 0 0 0 0 0 0 0 0
==> lib/librarylist.txt <== lib/new_similarity_NReagent_ReviR-OpenDEL0001.txt lib/new_similarity_NReagent_ReviR-OpenDEL0002.txt lib/new_similarity_NReagent_ReviR-OpenDEL0003.txt lib/new_similarity_NReagent_ReviR-OpenDEL0004.txt lib/new_similarity_NReagent_ReviR-OpenDEL0005.txt lib/new_similarity_NReagent_ReviR-OpenDEL0006.txt lib/new_similarity_NReagent_ReviR-OpenDEL0007.txt lib/new_similarity_NReagent_ReviR-OpenDEL0008.txt lib/new_similarity_NReagent_ReviR-OpenDEL0009.txt lib/new_similarity_NReagent_ReviR-OpenDEL0010.txt
Many thanks for your help!
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAV6ZR4Y7BR27VKFC2TWWRMGLANCNFSM6AAAAAAUWAFVGA . You are receiving this because you are subscribed to this thread.Message ID: @.***>
— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @.***>
从网易163邮箱发来的超大附件推荐客户端极速下载 Reference.zip (14.23M, 2023年2月24日 12:21 到期) 在线预览 | 下载 summarybyDNACodon.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 summarybyHitIndex.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 alignment.bam (56.04M, 2023年2月24日 12:22 到期) 下载
Hi Daniel. The links do not work. The hyperlink is missing in this email.
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @.> @.
发件人: daniel_deng @.*** 发送时间: 2023年2月9日 12:23 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Yes, here is the files. Thanks a lot for your attention.
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.>
At 2023-02-09 11:07:36, "LiYouBioinfo" @. <mailto:@.> > wrote:
Hi Daniel, could you send me the “Reference” folder and also the sam file in Elution-3 sample (under Analysis/) for me to debug?
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> > @. <mailto:@.>
发件人: daniel_deng @. <mailto:@.> 发送时间: 2023年2月9日 10:57 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: Subscribed 主题: [EXTERNAL][LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
I got error when running java -jar DEL_AnalysisToolkit_NAR.jar project -proj exp_2023020818:
[2023/02/08 17:48:07] ----1D1D-------- [102/372] [2023/02/08 17:48:38] ----1D1D----1R-- [2023/02/08 17:48:38] ----1D1D----1D-- [2023/02/08 17:49:00] ----2R1D----1D-- [2023/02/08 17:49:00] ----2D1D----1D-- [2023/02/08 17:49:11] ----2D1D----1D1R [2023/02/08 17:49:11] ----2D1D----1D1D [2023/02/08 17:49:19] ----3R1D----1D1D Fail to run molcal for Elution-3
cat step3.stderr
Exception in thread "main" java.lang.NullPointerException at DEL_AnalysisToolkit.DEL_Reads2Compounds.runMolCal(DEL_Reads2Compounds.java:384) at DEL_AnalysisToolkit.DEL_AnalysisToolkit.main(DEL_AnalysisToolkit.java:65)
My environment:
Ubuntu 18.04.6 LTS R version 4.2.0 (2022-04-22) openjdk version "1.8.0_312"
library('ggplot2') library('reshape2') library('gridExtra')
Library Preparation
Libraries were prepared using OpenDeL: java -jar DEL_AnalysisToolkit_NAR.jar convopendel -f lib_path.spapb -outdir lib library index files were placed in samples directory:
==> lib/librarylist.expID.txt <== 0 0 0 0 0 0 0 0 0 0
==> lib/librarylist.txt <== lib/new_similarity_NReagent_ReviR-OpenDEL0001.txt lib/new_similarity_NReagent_ReviR-OpenDEL0002.txt lib/new_similarity_NReagent_ReviR-OpenDEL0003.txt lib/new_similarity_NReagent_ReviR-OpenDEL0004.txt lib/new_similarity_NReagent_ReviR-OpenDEL0005.txt lib/new_similarity_NReagent_ReviR-OpenDEL0006.txt lib/new_similarity_NReagent_ReviR-OpenDEL0007.txt lib/new_similarity_NReagent_ReviR-OpenDEL0008.txt lib/new_similarity_NReagent_ReviR-OpenDEL0009.txt lib/new_similarity_NReagent_ReviR-OpenDEL0010.txt
Many thanks for your help!
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAV6ZR4Y7BR27VKFC2TWWRMGLANCNFSM6AAAAAAUWAFVGA . You are receiving this because you are subscribed to this thread.Message ID: @. <mailto:@.> >
— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @. <mailto:@.> >
从网易163邮箱发来的超大附件推荐客户端极速下载 Reference.zip (14.23M, 2023年2月24日 12:21 到期) 在线预览 | 下载 summarybyDNACodon.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 summarybyHitIndex.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 alignment.bam (56.04M, 2023年2月24日 12:22 到期) 下载
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3#issuecomment-1423610563 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAUDZTBPOYR4GB5UI2LWWRWMBANCNFSM6AAAAAAUWAFVGA . You are receiving this because you commented. https://github.com/notifications/beacon/AYAWWAQU4OKMVFLGYHLNDODWWRWMBA5CNFSM6AAAAAAUWAFVGCWGG33NNVSW45C7OR4XAZNMJFZXG5LFINXW23LFNZ2KUY3PNVWWK3TUL5UWJTSU3KJMG.gif Message ID: @. @.> >
Hi, Dr Li. It seems the attachment I sent to reply.github.com didn't work. Could you provide me with your personal email address? I will resend the files. Thanks!
--
Daniel Deng Cell: 15521321947 E-mail: @.***
在 2023-02-09 13:09:11,"LiYouBioinfo" @.***> 写道:
Hi Daniel. The links do not work. The hyperlink is missing in this email.
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @.> @.
发件人: daniel_deng @.*** 发送时间: 2023年2月9日 12:23 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Yes, here is the files. Thanks a lot for your attention.
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.>
At 2023-02-09 11:07:36, "LiYouBioinfo" @. <mailto:@.> > wrote:
Hi Daniel, could you send me the “Reference” folder and also the sam file in Elution-3 sample (under Analysis/) for me to debug?
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> > @. <mailto:@.>
发件人: daniel_deng @. <mailto:@.> 发送时间: 2023年2月9日 10:57 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: Subscribed 主题: [EXTERNAL][LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
I got error when running java -jar DEL_AnalysisToolkit_NAR.jar project -proj exp_2023020818:
[2023/02/08 17:48:07] ----1D1D-------- [102/372] [2023/02/08 17:48:38] ----1D1D----1R-- [2023/02/08 17:48:38] ----1D1D----1D-- [2023/02/08 17:49:00] ----2R1D----1D-- [2023/02/08 17:49:00] ----2D1D----1D-- [2023/02/08 17:49:11] ----2D1D----1D1R [2023/02/08 17:49:11] ----2D1D----1D1D [2023/02/08 17:49:19] ----3R1D----1D1D Fail to run molcal for Elution-3
cat step3.stderr
Exception in thread "main" java.lang.NullPointerException at DEL_AnalysisToolkit.DEL_Reads2Compounds.runMolCal(DEL_Reads2Compounds.java:384) at DEL_AnalysisToolkit.DEL_AnalysisToolkit.main(DEL_AnalysisToolkit.java:65)
My environment:
Ubuntu 18.04.6 LTS R version 4.2.0 (2022-04-22) openjdk version "1.8.0_312"
library('ggplot2') library('reshape2') library('gridExtra')
Library Preparation
Libraries were prepared using OpenDeL: java -jar DEL_AnalysisToolkit_NAR.jar convopendel -f lib_path.spapb -outdir lib library index files were placed in samples directory:
==> lib/librarylist.expID.txt <== 0 0 0 0 0 0 0 0 0 0
==> lib/librarylist.txt <== lib/new_similarity_NReagent_ReviR-OpenDEL0001.txt lib/new_similarity_NReagent_ReviR-OpenDEL0002.txt lib/new_similarity_NReagent_ReviR-OpenDEL0003.txt lib/new_similarity_NReagent_ReviR-OpenDEL0004.txt lib/new_similarity_NReagent_ReviR-OpenDEL0005.txt lib/new_similarity_NReagent_ReviR-OpenDEL0006.txt lib/new_similarity_NReagent_ReviR-OpenDEL0007.txt lib/new_similarity_NReagent_ReviR-OpenDEL0008.txt lib/new_similarity_NReagent_ReviR-OpenDEL0009.txt lib/new_similarity_NReagent_ReviR-OpenDEL0010.txt
Many thanks for your help!
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAV6ZR4Y7BR27VKFC2TWWRMGLANCNFSM6AAAAAAUWAFVGA . You are receiving this because you are subscribed to this thread.Message ID: @. <mailto:@.> >
— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @. <mailto:@.> >
从网易163邮箱发来的超大附件推荐客户端极速下载 Reference.zip (14.23M, 2023年2月24日 12:21 到期) 在线预览 | 下载 summarybyDNACodon.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 summarybyHitIndex.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 alignment.bam (56.04M, 2023年2月24日 12:22 到期) 下载
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3#issuecomment-1423610563 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAUDZTBPOYR4GB5UI2LWWRWMBANCNFSM6AAAAAAUWAFVGA . You are receiving this because you commented. https://github.com/notifications/beacon/AYAWWAQU4OKMVFLGYHLNDODWWRWMBA5CNFSM6AAAAAAUWAFVGCWGG33NNVSW45C7OR4XAZNMJFZXG5LFINXW23LFNZ2KUY3PNVWWK3TUL5UWJTSU3KJMG.gif Message ID: @. @.> >
— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @.***>
Hi Daniel. Please send the email to @. @.> . Thanks.
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @.> @.
发件人: daniel_deng @.*** 发送时间: 2023年2月9日 14:11 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Hi, Dr Li. It seems the attachment I sent to reply.github.com didn't work. Could you provide me with your personal email address? I will resend the files. Thanks!
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.>
在 2023-02-09 13:09:11,"LiYouBioinfo" @. <mailto:@.> > 写道:
Hi Daniel. The links do not work. The hyperlink is missing in this email.
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> > @. <mailto:@.>
发件人: daniel_deng @. <mailto:@.> 发送时间: 2023年2月9日 12:23 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Yes, here is the files. Thanks a lot for your attention.
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.> mailto:***@***.***
At 2023-02-09 11:07:36, "LiYouBioinfo" @. <mailto:@.> mailto:***@***.*** > wrote:
Hi Daniel, could you send me the “Reference” folder and also the sam file in Elution-3 sample (under Analysis/) for me to debug?
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> mailto:***@***.*** > @. <mailto:@.> mailto:***@***.***
发件人: daniel_deng @. <mailto:@.> mailto:***@***.*** 发送时间: 2023年2月9日 10:57 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: Subscribed 主题: [EXTERNAL][LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
I got error when running java -jar DEL_AnalysisToolkit_NAR.jar project -proj exp_2023020818:
[2023/02/08 17:48:07] ----1D1D-------- [102/372] [2023/02/08 17:48:38] ----1D1D----1R-- [2023/02/08 17:48:38] ----1D1D----1D-- [2023/02/08 17:49:00] ----2R1D----1D-- [2023/02/08 17:49:00] ----2D1D----1D-- [2023/02/08 17:49:11] ----2D1D----1D1R [2023/02/08 17:49:11] ----2D1D----1D1D [2023/02/08 17:49:19] ----3R1D----1D1D Fail to run molcal for Elution-3
cat step3.stderr
Exception in thread "main" java.lang.NullPointerException at DEL_AnalysisToolkit.DEL_Reads2Compounds.runMolCal(DEL_Reads2Compounds.java:384) at DEL_AnalysisToolkit.DEL_AnalysisToolkit.main(DEL_AnalysisToolkit.java:65)
My environment:
Ubuntu 18.04.6 LTS R version 4.2.0 (2022-04-22) openjdk version "1.8.0_312"
library('ggplot2') library('reshape2') library('gridExtra')
Library Preparation
Libraries were prepared using OpenDeL: java -jar DEL_AnalysisToolkit_NAR.jar convopendel -f lib_path.spapb -outdir lib library index files were placed in samples directory:
==> lib/librarylist.expID.txt <== 0 0 0 0 0 0 0 0 0 0
==> lib/librarylist.txt <== lib/new_similarity_NReagent_ReviR-OpenDEL0001.txt lib/new_similarity_NReagent_ReviR-OpenDEL0002.txt lib/new_similarity_NReagent_ReviR-OpenDEL0003.txt lib/new_similarity_NReagent_ReviR-OpenDEL0004.txt lib/new_similarity_NReagent_ReviR-OpenDEL0005.txt lib/new_similarity_NReagent_ReviR-OpenDEL0006.txt lib/new_similarity_NReagent_ReviR-OpenDEL0007.txt lib/new_similarity_NReagent_ReviR-OpenDEL0008.txt lib/new_similarity_NReagent_ReviR-OpenDEL0009.txt lib/new_similarity_NReagent_ReviR-OpenDEL0010.txt
Many thanks for your help!
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAV6ZR4Y7BR27VKFC2TWWRMGLANCNFSM6AAAAAAUWAFVGA . You are receiving this because you are subscribed to this thread.Message ID: @. <mailto:@.> mailto:***@***.*** >
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从网易163邮箱发来的超大附件推荐客户端极速下载 Reference.zip (14.23M, 2023年2月24日 12:21 到期) 在线预览 | 下载 summarybyDNACodon.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 summarybyHitIndex.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 alignment.bam (56.04M, 2023年2月24日 12:22 到期) 下载
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3#issuecomment-1423610563 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAUDZTBPOYR4GB5UI2LWWRWMBANCNFSM6AAAAAAUWAFVGA . You are receiving this because you commented. https://github.com/notifications/beacon/AYAWWAQU4OKMVFLGYHLNDODWWRWMBA5CNFSM6AAAAAAUWAFVGCWGG33NNVSW45C7OR4XAZNMJFZXG5LFINXW23LFNZ2KUY3PNVWWK3TUL5UWJTSU3KJMG.gif Message ID: @. <mailto:@.> @. <mailto:@.> > >
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Dear Dr Li, It seems github block email address as sensitive information. Could send me a message this address then I can reply.
--
Daniel Deng Cell: 15521321947 E-mail: @.***
At 2023-02-09 14:13:07, "LiYouBioinfo" @.***> wrote:
Hi Daniel. Please send the email to @. @.> . Thanks.
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @.> @.
发件人: daniel_deng @.*** 发送时间: 2023年2月9日 14:11 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Hi, Dr Li. It seems the attachment I sent to reply.github.com didn't work. Could you provide me with your personal email address? I will resend the files. Thanks!
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.>
在 2023-02-09 13:09:11,"LiYouBioinfo" @. <mailto:@.> > 写道:
Hi Daniel. The links do not work. The hyperlink is missing in this email.
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> > @. <mailto:@.>
发件人: daniel_deng @. <mailto:@.> 发送时间: 2023年2月9日 12:23 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Yes, here is the files. Thanks a lot for your attention.
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.> mailto:***@***.***
At 2023-02-09 11:07:36, "LiYouBioinfo" @. <mailto:@.> mailto:***@***.*** > wrote:
Hi Daniel, could you send me the “Reference” folder and also the sam file in Elution-3 sample (under Analysis/) for me to debug?
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> mailto:***@***.*** > @. <mailto:@.> mailto:***@***.***
发件人: daniel_deng @. <mailto:@.> mailto:***@***.*** 发送时间: 2023年2月9日 10:57 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: Subscribed 主题: [EXTERNAL][LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
I got error when running java -jar DEL_AnalysisToolkit_NAR.jar project -proj exp_2023020818:
[2023/02/08 17:48:07] ----1D1D-------- [102/372] [2023/02/08 17:48:38] ----1D1D----1R-- [2023/02/08 17:48:38] ----1D1D----1D-- [2023/02/08 17:49:00] ----2R1D----1D-- [2023/02/08 17:49:00] ----2D1D----1D-- [2023/02/08 17:49:11] ----2D1D----1D1R [2023/02/08 17:49:11] ----2D1D----1D1D [2023/02/08 17:49:19] ----3R1D----1D1D Fail to run molcal for Elution-3
cat step3.stderr
Exception in thread "main" java.lang.NullPointerException at DEL_AnalysisToolkit.DEL_Reads2Compounds.runMolCal(DEL_Reads2Compounds.java:384) at DEL_AnalysisToolkit.DEL_AnalysisToolkit.main(DEL_AnalysisToolkit.java:65)
My environment:
Ubuntu 18.04.6 LTS R version 4.2.0 (2022-04-22) openjdk version "1.8.0_312"
library('ggplot2') library('reshape2') library('gridExtra')
Library Preparation
Libraries were prepared using OpenDeL: java -jar DEL_AnalysisToolkit_NAR.jar convopendel -f lib_path.spapb -outdir lib library index files were placed in samples directory:
==> lib/librarylist.expID.txt <== 0 0 0 0 0 0 0 0 0 0
==> lib/librarylist.txt <== lib/new_similarity_NReagent_ReviR-OpenDEL0001.txt lib/new_similarity_NReagent_ReviR-OpenDEL0002.txt lib/new_similarity_NReagent_ReviR-OpenDEL0003.txt lib/new_similarity_NReagent_ReviR-OpenDEL0004.txt lib/new_similarity_NReagent_ReviR-OpenDEL0005.txt lib/new_similarity_NReagent_ReviR-OpenDEL0006.txt lib/new_similarity_NReagent_ReviR-OpenDEL0007.txt lib/new_similarity_NReagent_ReviR-OpenDEL0008.txt lib/new_similarity_NReagent_ReviR-OpenDEL0009.txt lib/new_similarity_NReagent_ReviR-OpenDEL0010.txt
Many thanks for your help!
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAV6ZR4Y7BR27VKFC2TWWRMGLANCNFSM6AAAAAAUWAFVGA . You are receiving this because you are subscribed to this thread.Message ID: @. <mailto:@.> mailto:***@***.*** >
— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @. <mailto:@.> mailto:***@***.*** >
从网易163邮箱发来的超大附件推荐客户端极速下载 Reference.zip (14.23M, 2023年2月24日 12:21 到期) 在线预览 | 下载 summarybyDNACodon.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 summarybyHitIndex.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 alignment.bam (56.04M, 2023年2月24日 12:22 到期) 下载
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3#issuecomment-1423610563 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAUDZTBPOYR4GB5UI2LWWRWMBANCNFSM6AAAAAAUWAFVGA . You are receiving this because you commented. https://github.com/notifications/beacon/AYAWWAQU4OKMVFLGYHLNDODWWRWMBA5CNFSM6AAAAAAUWAFVGCWGG33NNVSW45C7OR4XAZNMJFZXG5LFINXW23LFNZ2KUY3PNVWWK3TUL5UWJTSU3KJMG.gif Message ID: @. <mailto:@.> @. <mailto:@.> > >
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— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @.***>
Hi Daniel. Please send the email to liyoubioinfo at foxmail dot com
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @.> @.
发件人: daniel_deng @.*** 发送时间: 2023年2月9日 14:37 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Dear Dr Li, It seems github block email address as sensitive information. Could send me a message this address then I can reply.
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.>
At 2023-02-09 14:13:07, "LiYouBioinfo" @. <mailto:@.> > wrote:
Hi Daniel. Please send the email to @. <mailto:@.> @. <mailto:@.> > . Thanks.
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> > @. <mailto:@.>
发件人: daniel_deng @. <mailto:@.> 发送时间: 2023年2月9日 14:11 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Hi, Dr Li. It seems the attachment I sent to reply.github.com didn't work. Could you provide me with your personal email address? I will resend the files. Thanks!
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.> mailto:***@***.***
在 2023-02-09 13:09:11,"LiYouBioinfo" @. <mailto:@.> mailto:***@***.*** > 写道:
Hi Daniel. The links do not work. The hyperlink is missing in this email.
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> mailto:***@***.*** > @. <mailto:@.> mailto:***@***.***
发件人: daniel_deng @. <mailto:@.> mailto:***@***.*** 发送时间: 2023年2月9日 12:23 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: LiYouBioinfo; Comment 主题: [EXTERNAL]Re: [LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
Yes, here is the files. Thanks a lot for your attention.
--
Daniel Deng Cell: 15521321947 E-mail: @. <mailto:@.> mailto:***@***.*** mailto:***@***.***
At 2023-02-09 11:07:36, "LiYouBioinfo" @. <mailto:@.> mailto:***@***.*** mailto:***@***.*** > wrote:
Hi Daniel, could you send me the “Reference” folder and also the sam file in Elution-3 sample (under Analysis/) for me to debug?
Cheers
You Li (Lynn), Ph.D | 李游博士
Director of NGS and Bioinformatics | 测序与生物信息学总监
Lead Generation Unit | 先导化合物发现中心
HitGen Inc. | 成都先导药物开发股份有限公司
Building 6, Tianfu International Bio-Town,
No.8, Huigu 1st East Road, Shuangliu District,
Chengdu, Sichuan, China, 610000
中国四川成都市双流区(天府国际生物城)慧谷东一路8号6栋
O: +86-28-85197385-8215 | E: @. <mailto:@.> mailto:***@***.*** mailto:***@***.*** > @. <mailto:@.> mailto:***@***.*** mailto:***@***.***
发件人: daniel_deng @. <mailto:@.> mailto:***@***.*** mailto:***@***.*** 发送时间: 2023年2月9日 10:57 收件人: LiYouBioinfo/DELTA-Toolkit 抄送: Subscribed 主题: [EXTERNAL][LiYouBioinfo/DELTA-Toolkit] Fail to run molcal (Issue #3)
I got error when running java -jar DEL_AnalysisToolkit_NAR.jar project -proj exp_2023020818:
[2023/02/08 17:48:07] ----1D1D-------- [102/372] [2023/02/08 17:48:38] ----1D1D----1R-- [2023/02/08 17:48:38] ----1D1D----1D-- [2023/02/08 17:49:00] ----2R1D----1D-- [2023/02/08 17:49:00] ----2D1D----1D-- [2023/02/08 17:49:11] ----2D1D----1D1R [2023/02/08 17:49:11] ----2D1D----1D1D [2023/02/08 17:49:19] ----3R1D----1D1D Fail to run molcal for Elution-3
cat step3.stderr
Exception in thread "main" java.lang.NullPointerException at DEL_AnalysisToolkit.DEL_Reads2Compounds.runMolCal(DEL_Reads2Compounds.java:384) at DEL_AnalysisToolkit.DEL_AnalysisToolkit.main(DEL_AnalysisToolkit.java:65)
My environment:
Ubuntu 18.04.6 LTS R version 4.2.0 (2022-04-22) openjdk version "1.8.0_312"
library('ggplot2') library('reshape2') library('gridExtra')
Library Preparation
Libraries were prepared using OpenDeL: java -jar DEL_AnalysisToolkit_NAR.jar convopendel -f lib_path.spapb -outdir lib library index files were placed in samples directory:
==> lib/librarylist.expID.txt <== 0 0 0 0 0 0 0 0 0 0
==> lib/librarylist.txt <== lib/new_similarity_NReagent_ReviR-OpenDEL0001.txt lib/new_similarity_NReagent_ReviR-OpenDEL0002.txt lib/new_similarity_NReagent_ReviR-OpenDEL0003.txt lib/new_similarity_NReagent_ReviR-OpenDEL0004.txt lib/new_similarity_NReagent_ReviR-OpenDEL0005.txt lib/new_similarity_NReagent_ReviR-OpenDEL0006.txt lib/new_similarity_NReagent_ReviR-OpenDEL0007.txt lib/new_similarity_NReagent_ReviR-OpenDEL0008.txt lib/new_similarity_NReagent_ReviR-OpenDEL0009.txt lib/new_similarity_NReagent_ReviR-OpenDEL0010.txt
Many thanks for your help!
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAV6ZR4Y7BR27VKFC2TWWRMGLANCNFSM6AAAAAAUWAFVGA . You are receiving this because you are subscribed to this thread.Message ID: @. <mailto:@.> mailto:***@***.*** mailto:***@***.*** >
— Reply to this email directly, view it on GitHub, or unsubscribe. You are receiving this because you authored the thread.Message ID: @. <mailto:@.> mailto:***@***.*** mailto:***@***.*** >
从网易163邮箱发来的超大附件推荐客户端极速下载 Reference.zip (14.23M, 2023年2月24日 12:21 到期) 在线预览 | 下载 summarybyDNACodon.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 summarybyHitIndex.txt.gz (10B, 2023年2月24日 12:21 到期) 下载 alignment.bam (56.04M, 2023年2月24日 12:22 到期) 下载
— Reply to this email directly, view it on GitHub https://github.com/LiYouBioinfo/DELTA-Toolkit/issues/3#issuecomment-1423610563 , or unsubscribe https://github.com/notifications/unsubscribe-auth/AYAWWAUDZTBPOYR4GB5UI2LWWRWMBANCNFSM6AAAAAAUWAFVGA . You are receiving this because you commented. https://github.com/notifications/beacon/AYAWWAQU4OKMVFLGYHLNDODWWRWMBA5CNFSM6AAAAAAUWAFVGCWGG33NNVSW45C7OR4XAZNMJFZXG5LFINXW23LFNZ2KUY3PNVWWK3TUL5UWJTSU3KJMG.gif Message ID: @. <mailto:@.> mailto:***@***.*** @. <mailto:@.> mailto:***@***.*** > >
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I got error when running
java -jar DEL_AnalysisToolkit_NAR.jar project -proj exp_2023020818
:cat step3.stderr
My environment:
Library Preparation
Libraries were prepared using OpenDeL:
java -jar DEL_AnalysisToolkit_NAR.jar convopendel -f lib_path.spapb -outdir lib
library index files were placed in samples directory:Many thanks for your help!