Closed lcolladotor closed 2 years ago
01_
09_snRNA-seq_re-processed
s3e.hb
emptyDrops()
Rdata
reference_gtf
/dcs04/lieber/lcolladotor/annotationFiles_LIBD001/10x/refdata-gex-GRCh38-2020-A/enes/genes.gtf
/dcl01/ajaffe/data/lab/singleCell/cellranger_reference/refdata-gex-GRCh38-2020-A/genes/genes.gtf
dcs04
match()
s3e.hb$cellType
sce$cellType_Erik
sce
sgejobs::job_single()
qsub
02_qc.R
01_
prefix) at09_snRNA-seq_re-processed
s3e.hb
. Choose something easy to type ^^. Aka, you won't need https://github.com/LieberInstitute/Habenula_Bulk/blob/master/code/09_snRNA-seq_re-processed/01_qc.R#L42 anymore.emptyDrops()
output into it's ownRdata
file https://github.com/LieberInstitute/Habenula_Bulk/blob/master/code/08_snRNA-seq_Erik/20220228_human_hb_neun.R#L108reference_gtf
https://github.com/LieberInstitute/spatialLIBD/blob/master/R/read10xVisiumWrapper.R#L88 can be/dcs04/lieber/lcolladotor/annotationFiles_LIBD001/10x/refdata-gex-GRCh38-2020-A/enes/genes.gtf
taken from http://research.libd.org/spatialLIBD/articles/TenX_data_download.html#from-10x or you could use the exact same one Erik used at https://github.com/LieberInstitute/Habenula_Bulk/blob/master/code/08_snRNA-seq_Erik/20210323_human_hb_neun.R#L197 which is/dcl01/ajaffe/data/lab/singleCell/cellranger_reference/refdata-gex-GRCh38-2020-A/genes/genes.gtf
. Both files are in reality identical and I would encourage that you use the one fromdcs04
.match()
https://github.com/LieberInstitute/Habenula_Bulk/blob/master/code/09_snRNA-seq_re-processed/01_qc.R#L49 and how things are assigned at https://github.com/LieberInstitute/Habenula_Bulk/blob/master/code/09_snRNA-seq_re-processed/01_qc.R#L50-L53.s3e.hb$cellType
) assce$cellType_Erik
or something like that (changesce
for whatever name you used earlier).sgejobs::job_single()
to add a shell script if you want to be able toqsub
this new R script. You can then have a single shell script for re-running everything if needed, like https://github.com/LieberInstitute/Visium_IF_AD/blob/master/code/run_all_post_spaceranger.sh for example.02_qc.R
.emptyDrops()
) instead of Erik's at https://github.com/LieberInstitute/Habenula_Bulk/blob/master/code/09_snRNA-seq_re-processed/01_qc.R#L7.