Determining average DAPI+ nuclei per spot

LieberInstitute / Visium_SPG_AD

Visium SPG AD project (n = 10) using Visium Spatial Proteogenomics (Visium-SPG) on dissections from the inferior temporal cortex (ITC) from Alzheimer's disease cases and controls.

https://research.libd.org/Visium_SPG_AD

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Determining average DAPI+ nuclei per spot #78

Closed shkwon17 closed 1 year ago

shkwon17 commented 1 year ago

"...we observed XXX nuclei per spot in the ITC tissue. In addition, since spots are aligned in a honeycomb pattern with 100µm center-to-center distance between neighboring spots, there is 45µm of inter-spot space where transcriptomic data is not captured 45."

shkwon17 commented 1 year ago

https://jhu-genomics.slack.com/archives/C01GJ0F731Q/p1677859988407809

sample_id average_NDAPI

1 S1_A1_Br3874 3.35 2 S1_B1_Br3854 3.82 3 S1_C1_Br3873 4.73 4 S1_D1_Br3880 5.51 5 S2_A1_Br3874 4.13 6 S2_B1_Br3854 3.98 7 S2_C1_Br3873 4.65 8 S2_D1_Br3880 5.37 9 S3_A1_Br3874 3.41 10 S3_D1_Br3880 6.12