LieberInstitute / spatialDLPFC

spatialDLPFC project involving Visium (n = 30), Visium SPG (n = 4) and snRNA-seq (n = 19) samples
http://research.libd.org/spatialDLPFC/
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differential expression analysis between regions of dlpfc #63

Closed abspangler13 closed 2 years ago

abspangler13 commented 2 years ago

first part of this: https://jhu-genomics.slack.com/archives/C01EA7VDJNT/p1648751711386829 zoom: https://jhu-genomics.slack.com/archives/C029NB48G9K/p1648831514251679

usually use limma to do differential expression or we can use pseudobulk dge. this chapter of osca http://bioconductor.org/books/3.14/OSCA.workflows/segerstolpe-human-pancreas-smart-seq2.html#segerstolpe-comparison