LieberInstitute / spatialLIBD

Code for the spatialLIBD R/Bioconductor package and shiny app
http://LieberInstitute.github.io/spatialLIBD/
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problem with read10xVisiumWrapper function #38

Closed abspangler13 closed 2 years ago

abspangler13 commented 2 years ago

Unable to run read10xVisiumWrapper function without specifying reference_gtf argument.

Context

The error we get is this:

Screen Shot 2022-04-08 at 1 36 27 PM

R session information

options(width = 120)
R Under development (unstable) (2021-11-06 r81149)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: CentOS Linux 7 (Core)
Matrix products: default
BLAS:   /jhpce/shared/jhpce/core/conda/miniconda3-4.6.14/envs/svnR-devel/R/devel/lib64/R/lib/libRblas.so
LAPACK: /jhpce/shared/jhpce/core/conda/miniconda3-4.6.14/envs/svnR-devel/R/devel/lib64/R/lib/libRlapack.so
locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C
 [9] LC_ADDRESS=C               LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats4    stats     graphics  grDevices datasets  utils     methods
[8] base
other attached packages:
 [1] sessioninfo_1.2.2           lobstr_1.1.1
 [3] rtracklayer_1.55.4          spatialLIBD_1.7.12
 [5] SpatialExperiment_1.5.3     SingleCellExperiment_1.17.2
 [7] SummarizedExperiment_1.25.3 Biobase_2.55.1
 [9] GenomicRanges_1.47.6        GenomeInfoDb_1.31.7
[11] IRanges_2.29.1              S4Vectors_0.33.17
[13] BiocGenerics_0.41.2         MatrixGenerics_1.7.0
[15] matrixStats_0.61.0          here_1.0.1
loaded via a namespace (and not attached):
  [1] spam_2.8-0                    AnnotationHub_3.3.9
  [3] BiocFileCache_2.3.4           lazyeval_0.2.2
  [5] Polychrome_1.3.1              BiocParallel_1.29.20
  [7] scater_1.23.6                 usethis_2.1.5
  [9] ggplot2_3.3.5                 benchmarkme_1.0.7
 [11] digest_0.6.29                 foreach_1.5.2
 [13] htmltools_0.5.2               viridis_0.6.2
 [15] magick_2.7.3                  fansi_1.0.3
 [17] magrittr_2.0.3                memoise_2.0.1
 [19] ScaledMatrix_1.3.0            config_0.3.1
 [21] doParallel_1.0.17             limma_3.51.6
 [23] Biostrings_2.63.3             R.utils_2.11.0
 [25] colorspace_2.0-3              ggrepel_0.9.1
 [27] blob_1.2.2                    rappdirs_0.3.3
 [29] xfun_0.30                     dplyr_1.0.8
 [31] crayon_1.5.1                  RCurl_1.98-1.6
 [33] jsonlite_1.8.0                roxygen2_7.1.2
 [35] iterators_1.0.14              glue_1.6.2
 [37] gtable_0.3.0                  zlibbioc_1.41.0
 [39] XVector_0.35.0                DelayedArray_0.21.2
 [41] BiocSingular_1.11.0           DropletUtils_1.15.2
 [43] Rhdf5lib_1.17.3               maps_3.4.0
 [45] HDF5Array_1.23.2              scales_1.1.1
 [47] DBI_1.1.2                     edgeR_3.37.1
 [49] Rcpp_1.0.8.3                  viridisLite_0.4.0
 [51] xtable_1.8-4                  dqrng_0.3.0
 [53] rsvd_1.0.5                    bit_4.0.4
 [55] dotCall64_1.0-1               DT_0.22
 [57] htmlwidgets_1.5.4             httr_1.4.2
 [59] RColorBrewer_1.1-3            ellipsis_0.3.2
 [61] XML_3.99-0.9                  pkgconfig_2.0.3
 [63] R.methodsS3_1.8.1             scuttle_1.5.1
 [65] sass_0.4.1                    dbplyr_2.1.1
 [67] locfit_1.5-9.5                utf8_1.2.2
 [69] tidyselect_1.1.2              rlang_1.0.2
 [71] later_1.3.0                   AnnotationDbi_1.57.1
 [73] munsell_0.5.0                 BiocVersion_3.15.2
 [75] tools_4.2.0                   cachem_1.0.6
 [77] cli_3.2.0                     generics_0.1.2
 [79] RSQLite_2.2.12                ExperimentHub_2.3.5
 [81] stringr_1.4.0                 fastmap_1.1.0
 [83] yaml_2.3.5                    knitr_1.38
 [85] bit64_4.0.5                   fs_1.5.2
 [87] purrr_0.3.4                   KEGGREST_1.35.0
 [89] sparseMatrixStats_1.7.0       mime_0.12
 [91] R.oo_1.24.0                   xml2_1.3.3
 [93] rstudioapi_0.13               brio_1.1.3
 [95] compiler_4.2.0                beeswarm_0.4.0
 [97] plotly_4.10.0                 filelock_1.0.2
 [99] curl_4.3.2                    png_0.1-7
[101] interactiveDisplayBase_1.33.0 testthat_3.1.3
[103] tibble_3.1.6                  bslib_0.3.1
[105] stringi_1.7.6                 desc_1.4.1
[107] fields_13.3                   lattice_0.20-45
[109] Matrix_1.4-1                  vctrs_0.4.0
[111] pillar_1.7.0                  lifecycle_1.0.1
[113] rhdf5filters_1.7.0            BiocManager_1.30.16
[115] jquerylib_0.1.4               BiocNeighbors_1.13.0
[117] irlba_2.3.5                   data.table_1.14.2
[119] cowplot_1.1.1                 bitops_1.0-7
[121] httpuv_1.6.5                  BiocIO_1.5.0
[123] R6_2.5.1                      promises_1.2.0.1
[125] gridExtra_2.3                 vipor_0.4.5
[127] codetools_0.2-18              benchmarkmeData_1.0.4
[129] assertthat_0.2.1              pkgload_1.2.4
[131] golem_0.3.2                   rhdf5_2.39.6
[133] rprojroot_2.0.3               rjson_0.2.21
[135] shinyWidgets_0.6.4            withr_2.5.0
[137] GenomicAlignments_1.31.2      Rsamtools_2.11.0
[139] GenomeInfoDbData_1.2.7        parallel_4.2.0
[141] grid_4.2.0                    beachmat_2.11.0
[143] tidyr_1.2.0                   attempt_0.3.1
[145] DelayedMatrixStats_1.17.0     scatterplot3d_0.3-41
[147] shiny_1.7.1                   ggbeeswarm_0.6.0
[149] restfulr_0.0.13
lcolladotor commented 2 years ago

v1.7.15 has resolved this issue, thanks for the report @abspangler13 @heenadivecha!