HPC dissections have been performed for Br6432, Br6423, Br2743, Br2720 and Br3942. For all 5 of these brains, slides have been made for QC by H&E staining and RNAscope for the 4 subfield markers. Additionally, 5 100um sections were collected prior to (anterior to) the QC slides to be reserved for snRNAseq.
To minimize the potential for number transposition errors causing confusion, we added a letter to each BrNum, going in rainbow color order (red, orange, yellow, green, blue, indigo, violet, pink, white, silver) like:
Br6432R
Br6423O
Br2743Y
Br2720G
Br3942B
and additional brains will be lettered I, V, P, W, S.
Remaining dissections need to be scheduled with Neuropath @stephaniecpage
HPC dissections have been performed for Br6432, Br6423, Br2743, Br2720 and Br3942. For all 5 of these brains, slides have been made for QC by H&E staining and RNAscope for the 4 subfield markers. Additionally, 5 100um sections were collected prior to (anterior to) the QC slides to be reserved for snRNAseq.
To minimize the potential for number transposition errors causing confusion, we added a letter to each BrNum, going in rainbow color order (red, orange, yellow, green, blue, indigo, violet, pink, white, silver) like:
Remaining dissections need to be scheduled with Neuropath @stephaniecpage