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LomanLab / mockcommunity

Long-read mock community experiments
https://lomanlab.github.io/mockcommunity/
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species of R10 data #19

Closed yangxiaofeill closed 5 years ago

yangxiaofeill commented 5 years ago

Dear, I head a talk today, and the speaker introduced the R10, which is much more accurate than R9.4. I am interested in the R10 data and found this github site. I wonder what is the species of the data https://s3.climb.ac.uk/nanopore/Zymo-GridION-EVEN-BB-SN-PCR-R10HC-flipflop.fq.gz and https://s3.climb.ac.uk/nanopore/Zymo-GridION-EVEN-BB-SN-PCR-R10HC_multi.tar. And also, I would like to know the coverage of this dataset.

Thanks very much.

Best Xiaofei

SamStudio8 commented 5 years ago

Hi Xiaofei, The datasets in this project are sequencing runs of the ZymoBIOMICS Microbial Community Standard, which is a mock community consisting of ten organisms. Zymo-GridION-EVEN-BB-SN-PCR-R10HC-flipflop.fq.gz is the basecalled data from a sequencing run on the R10, and the Zymo-GridION-EVEN-BB-SN-PCR-R10HC_multi.tar is the corresponding multi-FAST5 signal.

The sequencing libraries were derived from @joshquick's "Samurai" PCR protocol.