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LooseLab / readfish

CLI tool for flexible and fast adaptive sampling on ONT sequencers
https://looselab.github.io/readfish/
GNU General Public License v3.0
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Which sequencing experiment generated the example Bulk Fast5 file? #166

Closed Huanle closed 2 years ago

Huanle commented 2 years ago

Hi readfish developers,

Can you tell me which sequencing experiment was performed to generate the fast5 file - PLSP57501_20170308_FNFAF14035_MN16458_sequencing_run_NOTT_Hum_wh1rs2_60428.fast5?
In another word, what was sequenced？ Thanks a lot in advance!

Huanle commented 2 years ago

I think i found the answer here. But it would be nice to get the bulk fast5 files for NB4 cell line and the ZymoBIOMICS sequencing experiments. Can you please let me know if this is possible?

mattloose commented 2 years ago

Hi, You are correct - the example bulk file is a sequencing run for NA12878. We provide that file as it did not itself have readfish run on it. We have a short bulk file available for ZymoBIOMICS which we might be able to make available if we can find an appropriate location to host it.

We will post a link here if we can.

Huanle commented 2 years ago

Hi @mattloose ,

Thanks a lot for your prompt reply. I look forward to having a try with the ZymoBIOMICS and NB4 cell line sequencing data.

Best,