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LooseLab / readfish

CLI tool for flexible and fast adaptive sampling on ONT sequencers
https://looselab.github.io/readfish/
GNU General Public License v3.0
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readfish not work #206

Closed windforclouds closed 1 year ago

windforclouds commented 2 years ago

we had some problems，when we open readfish, you can see this minknowcore 5.0.0 ; guppy 6.0.7 ; minknow 22.03.6 image

windforclouds commented 2 years ago

$cat human_chr_selection.toml [caller_settings] config_name = "dna_r9.4.1_450bps_fast" host = "ipc:///tmp/.guppy"

host = "127.0.0.1"

port = 5555

[conditions] reference = "/data/test/02.readfish/ref_hg19/ucsc.hg19_NC_012920.1.mmi"

[conditions.0] name = "select_chr_21_22" control = false min_chunks = 0 max_chunks = inf targets = "/data/test/02.readfish/rawdata/ontarget.txt" single_on = "stop_receiving" multi_on = "stop_receiving" single_off = "unblock" multi_off = "unblock" no_seq = "proceed" no_map = "proceed"

targets = ["chr21", "chr22"]

Adoni5 commented 1 year ago

Hi @windforclouds - Do you still need need help with this? Could you let me know what branch of the readfish repository you are working with? The immediate issue that I can spot is that MinKNOW no longer uses the 9501 port for GRPC, as this is the port is used to listen on for unencrypted connections, but instead listens on the 9502 port for secured connections.

mattloose commented 1 year ago

Closed as stale.