LooseLab / readfish

CLI tool for flexible and fast adaptive sampling on ONT sequencers
https://looselab.github.io/readfish/
GNU General Public License v3.0
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Multiplex bulk fast5 simulation on Promethion #247

Closed lkwhite closed 1 year ago

lkwhite commented 1 year ago

Thanks for providing all this documentation of running simulated sequencing runs using bulk fast5s. I'm currently trying to run simulations on a Promethion 24, which can generate and basecall many runs in parallel. Wondering if there's a tested way to specify multiple runs for simulation in a .toml file. Have you played around with this at all? And/or do you know if it's safe to run additional sequencing runs (as long as they are using different config files) while one is running a simulation at another position?

mattloose commented 1 year ago

You can create multiple toml files with different fast5 files and choose them when you start. You can also run the same simulation toml across many different flowcells.

You can run real sequencing alongside playback if you wish as well. But don't get confused with what is running on which position!