LooseLab / readfish

CLI tool for flexible and fast adaptive sampling on ONT sequencers
https://looselab.github.io/readfish/
GNU General Public License v3.0
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Ask about simuliation without Minknow device #249

Closed ElroyLR closed 1 year ago

ElroyLR commented 1 year ago

Hello, I'm looking forward to running readfish on some samples,So I tested the fast5 file provided. According to the description in the readme.md file, an attempt was made to add [custom_settings] and 'silumination='my/path/to/fast5' to the accompanying toml file in the repo, but an error occurred:

😾 This TOML file has failed validation See below for details:
Additional properties are not allowed ('custom_settings' was unexpected)

Then I modified the 'ru/static/readfish_toml. schema. json' file, but I couldn't customize the format of the toml file, and the above error still occurred.

So I have some questions,

  1. How to solve this problem?
  2. Can readfish use fast5 files for testing without connecting to Minknow devices?
  3. Do I have to use the sever version of Guppy to apply to readfish? Can Guppy installed locally be supported?

Have a good day. Looking forward to your reply.

alexomics commented 1 year ago

Close as duplicate of #248