Hi,
I have run a starchip for the detection of the circular RNA in my Ribodeplected RNA-SEQ datasets. The pipeline runs sucessfully and I got blank result at the step 4. When I have check R script, I found that the median ratio for the all the detected cirRNA was higher than 2 in all the sample. I want to understand about how the strandimbalance ratio is calculated and raitional behind it. Please share detailed explanation with exmaple if possible.
Thank you,
Hi, I have run a starchip for the detection of the circular RNA in my Ribodeplected RNA-SEQ datasets. The pipeline runs sucessfully and I got blank result at the step 4. When I have check R script, I found that the median ratio for the all the detected cirRNA was higher than 2 in all the sample. I want to understand about how the strandimbalance ratio is calculated and raitional behind it. Please share detailed explanation with exmaple if possible. Thank you,