Could not open file: index.dnascent

MBoemo / DNAscent

Software for detecting regions of BrdU and EdU incorporation in Oxford Nanopore reads.

https://www.boemogroup.org/

GNU General Public License v3.0

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Could not open file: index.dnascent #24

Closed rmayang28 closed 2 years ago

rmayang28 commented 2 years ago

Hi,

I have downloaded DNAscent and when running DNAscent command, I could see all the proper executables. But when I try to run ./DNAscent index -f /data/a/PEAP/pION/MinION/20220511_exp1_ku80/20220511_exp1/no_sample/20220511_1300_MN38257_FAR60553_a490da1d/fast5_pass/ I would get an error message:

terminate called after throwing an instance of 'IOerror'
  what():  Could not open file: index.dnascent
Aborted

Please let me know what I should do to try and fix this.

Thank you Mai

MBoemo commented 2 years ago

It looks like you don't have write permissions to the folder you're running DNAscent from, hence it can't write the index output file. I'd try creating a folder you know you can write to, and then run DNAscent index from there. Or, alternatively, you can specify the path of the output file you want with -o. Closing for now, but reopen if that doesn't fix it.