About Barcode correction

[10KGenomics]

Hello, I would like to ask whether this software can perform barcode correction. In one case, there are two kinds of beads in the oil drop, but only one cell. in this scenario, one cell will split its transcripts across two barcodes and appear like two beads。 In this case, we should correct and combine the two barcodes to avoid errors.

[lishuangshuang0616]

A droplet contains two kinds of beads, and the functions of the two kinds of beads are different. We call them cell beads and index_carrier beads. The function of cell beads is to distinguish cells, and the function of index_carrier beads is to combine cell beads in a droplet (there will be not only one cell beads in one droplet, conforms to a Poisson distribution). We will perform barcode correction for cell beads and index_carrier beads (Hamming distance 1)。

[10KGenomics]

A droplet contains two kinds of beads, and the functions of the two kinds of beads are different. We call them cell beads and index_carrier beads. The function of cell beads is to distinguish cells, and the function of index_carrier beads is to combine cell beads in a droplet (there will be not only one cell beads in one droplet, conforms to a Poisson distribution). We will perform barcode correction for cell beads and index_carrier beads (Hamming distance 1)。

there are two kinds of cell beads in the oil drop, but only one cell. in this scenario, one cell will split its transcripts across two cell barcodes and appear like two cell beads. You mean index_ Carrier beads will correct the two cell beads to become a cell beads?