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MGI-tech-bioinformatics / DNBelab_C_Series_HT_scRNA-analysis-software

An open source and flexible pipeline to analysis high-throughput DNBelab C Series single-cell RNA datasets
MIT License
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alignment_report.csv 统计问题 #5

Closed cherriyoush closed 1 year ago

cherriyoush commented 1 year ago

你好，输出文件alignment_report.csv中的 Raw reads和Mapped reads一样，我跑的3个样本比对率都是100%: image 这个Raw reads 应该是文件Log.final.out中的行 Number of input reads的对应统计的reads数吧

lishuangshuang0616 commented 1 year ago

可以参考html报告中的结果，alignment_report.csv中Raw Reads和Mapped reads实际都为Mapped reads（生成的bam中去除了未比对上的reads减少bam存储），真实的Raw Reads结果见cDNA_sequencing_report.csv中的Fragments pass QC。

cherriyoush commented 1 year ago

多谢