dnbc4tools atac run 运行报错

[Ruvic-chen]

您好，我最近在singularity上运行dnbc4tools atac run遇到了报错，希望可以得到帮助，报错信息如下：

[lishuangshuang0616]

Based on your data, the software cannot recognize the sequence structure. Could you please provide screenshots of the first few sequences of R1 and R2?

[Ruvic-chen]

Based on your data, the software cannot recognize the sequence structure. Could you please provide screenshots of the first few sequences of R1 and R2?

这是R1的序列信息 这是R2的序列信息

[lishuangshuang0616]

Was your data mixed with the Illumina library and used APP-D reagent?

[hullv]

Was your data mixed with the Illumina library and used APP-D reagent?

hello, i meet the same error. If my data is mixed with the Illumina library and APP-D reagent is used, how should the data be processed?

[lishuangshuang0616]

The data cannot be analyzed because the cell barcode and umi sequences cannot be sequenced. @hullv

[hullv]

The data cannot be analyzed because the cell barcode and umi sequences cannot be sequenced. @hullv RNA cannot be analyzed? Can't ATAC be analyzed either?

[lishuangshuang0616]

scRNA has no effect and can be analyzed. scATAC cannot analyze. @hullv