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MGI-tech-bioinformatics / DNBelab_C_Series_HT_scRNA-analysis-software

An open source and flexible pipeline to analysis high-throughput DNBelab C Series single-cell RNA datasets
MIT License
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使用PE100模式测序是否适用DNBc4tools #75

Closed Tc940417 closed 4 months ago

Tc940417 commented 4 months ago

老师您好，我的文库使用了PE100的测序模式，咱们的工具可以自动截取序列格式吗？还是需要手动截取一下，谢谢！

lishuangshuang0616 commented 4 months ago

It can automatically trim and recognize if there is a dark reaction mode in PE100, finding the positions of CB and UMI. The software will analyze directly and automatically identify these positions. However, if there are multiple sequences with inconsistent lengths, manual trimming is required.

Tc940417 commented 4 months ago

thanks for your answer @lishuangshuang0616