Open ultraypy opened 1 month ago
Raw is the transcript expression matrix of all detected cell barcodes, and filter is the matrix identified as real cells after the background is filtered out by cell calling. It is recommended to use filter, you can also use raw to filter manually.
Can I use the Seurat workflow for filtering?
yes , use subset to filter ,subset gene,umi,mt etc
pbmc <- [subset](https://rdrr.io/r/base/subset.html)(pbmc, subset = nFeature_RNA > $num1 & nFeature_RNA < $num2 & percent.mt < $num3)
我可不可以自己对raw matrix进行预处理和过滤