Open peipp410 opened 4 weeks ago
Can you check the results in 01.data/oligo.sequencing.report.csv and also provide the screenshot of the results from 01.data/oligo.reads.fq.gz?
None of the reads passed QC, which is so wired. The fastq file is also empty. How could that happen?
p.s. with arguments "--chemistry scRNAv2HT --darkreaction unset,unset"
Screenshot the first 10 sequences R1/R2 of cDNA and oligo, I'll take a look
Thanks! Problem solved. 'N'-base exists.
Yes, this version will cause reads to be filtered for oligo fastq containing N. Currently, you can only manually remove the fixed sequence. This problem will be solved in the upcoming 2.1.3 version.
Hello! I encountered the following error. I tried to check the source code but I can't locate where the function is because there is no file called 'oligo_filter.py'. What's the possible cause of this error and how to fix that? Thanks!![WechatIMG2977](https://github.com/user-attachments/assets/1517cdee-494a-4064-a5f2-948c72fa644f)