One Hybrid for DBD protein / activator protein

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1 Hybrid

Interaction involving an Activator, can be done in mammals cells Protein A fused to DB Protein B (not fused already have a AD) If there is reporter transcription, you will know that (with the good controls) : protein A binds B The detection technics added is 'two hybrid' for the moment , but the protein B is not fused to an AD here. It is a complementation but between a tag on the bait and the prey ( in 2 hybrid: complementation between the tag of the bait and the tag of the prey)

The one in the CV: bait = DNA promoter, prey = DBD protein (with also an activation domain) -> DNA/protein interaction

The one to be added: bait= DBD protein, prey = activator -> DBD protein/activator protein interaction. Here DBD and activator are encoded by different cDNA

It is a bit different so a new term may be needed. But we may need to adapt each name.

PMID: 10871379 , 10629049

It differs from :

term: one hybrid id: MI:0432 definition: Protein-DNA Complementation assay where a single promoter act as bait and is screened against a library of prey transcription factors. definition_reference: PMID:10589421

Karine

Reported by: krobbe4

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PSI geneva meeting reject the addition of a new term for the first case of protein-transcription factor 1 hybrid described above. These experiments should be described as 2hybrid with some eventual extra annotation specifying that the bait is not engeneered. One Hybrid MI:0432 definition remains as it is.

Original comment by: luisa_montecchi

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dicussed that One hybrid term stays only for protein-DNA interactions no term added for one hybrid protein protein interaction. For this latter case consider the use of 2h terms with some text annotation specifying the implementation variation of the 2h experiment

Original comment by: luisa_montecchi

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• status: open --> closed

Original comment by: luisa_montecchi