IDM:...:biophysical:DSC

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child_of Interaction detection method (MI:0001) - experimental interaction detection (MI:0045) - biophysical (MI:0013)

Definition: Differential scanning calorimetry (DSC) is a thermoanalytical technique in which the difference in the amount of heat required to increase the temperature of a sample and reference in solution is measured as a function of temperature. By measuring the temperature dependence of this partial heat capacity, a basic thermodynamic property, DSC gives immediate access to the thermodynamic mechanism that governs a conformational equilibrium, for example between a protein complex and its individual participants. The system is heated (or cooled) quasi-adiabatically at a constant rate, typically 0.5 – 1.5 K min-1. Since the heat capacities of the solution in the sample cell and the solvent in the reference cell differ, a certain amount of electrical power is required to zero the temperature difference between the two cells. The power difference (J s-1), after normalization by the scanning rate (K s-1), is a direct measure of the heat capacity difference between the sample and the reference solution: dCp(sol – solv) = Cp(sol) – Cp(solv) (in units of J K-1).

PMID: 10398392

Reported by: bmeldal

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MI:1311

Original comment by: orchard

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Original comment by: orchard