supercharged green fluorescent protein tag

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GFP variants with mutated surfaces resulting in an altered net charge (PMID:17665911). For instance, negatively charged scGFP fusion proteins are used in native gel electrophoresis mobility shift experiments in PMID:21763699.

Reported by: kroey

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Can you not use MI:0734 (enhanced green fluorescent protein tag) for this. We do not state what the enhancements are. If not, let me know ASAP, as I have an updated CV ready for release and can add it to that.

Original comment by: orchard

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if eGFP means any variation of gfp then yes, I guess we can use MI:0734 for this tag...

On 20 Jan 2014, at 13:19, Sandra Orchard wrote:

Can you not use MI:0734 (enhanced green fluorescent protein tag) for this. We do not state what the enhancements are. If not, let me know ASAP, as I have an updated CV ready for release and can add it to that.

[mi-controlled-vocab-changes:#355] supercharged green fluorescent protein tag

Status: open Created: Mon Jan 20, 2014 08:28 AM UTC by kroey Last Updated: Mon Jan 20, 2014 08:28 AM UTC Owner: nobody

GFP variants with mutated surfaces resulting in an altered net charge (PMID:17665911). For instance, negatively charged scGFP fusion proteins are used in native gel electrophoresis mobility shift experiments in PMID:21763699.

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Original comment by: kroey

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Actually, have since been looking and eGFP refers to a very specific mutant and a very specific wavelength. I'll create a new term.

Original comment by: orchard

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MI:1338

Original comment by: orchard

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• status: open --> closed
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Original comment by: orchard