Closed felipealbrecht closed 4 years ago
Pou5f1, Sox2, Nanog are crucial factors for the pluripotency of ES cells.
Experiment SRX1053369
colocalization
Sox2: SRX512370 Nanog: SRX702041
Overlapping by CSS Strong Enhancers , BioSources H1-hESC and HUVEC cell. It is possible to see that these TFs are present in more abundances in the Strong Enhancers of the ESC cell line. GM12878 is derived from lymphoblast cells
Same for "Enh" state: Induced pluripotent stem cell skeletal muscle tisue stromal
Extend 2.5K to both directions Gene Ontology - check the genes
TFs as background EP300 universal activating in enhancers - background SRX027482
https://dive.mpi-inf.mpg.de/#/load_query?qid=q7694160&gid=g1&cid=q7694243&cid=q7694211
also load the EP300 (see above) dataset.
Find the regions that does not overlap and enrich these regions
The three previous factors are crucial for pluripotency
EZH2 and SUZ12 are transcriptor repressors the data
Compare these in the Pluripotent and differentiated cells.
In ES: The 3 factors are activated In oposite surpresed
in differentiated cells the kind of repressor proteins EZH2 and SUZ12 are binding new the genes in ES cells, because these genes are suppressed in differentiated cells
Find an example.
Plots with 6 datasets: 3 ESC 2 Repressors 1 EP300
Pou5f1 Sox2 Nanog