MSGFPlus / msgfplus

MS-GF+ (aka MSGF+ or MSGFPlus) performs peptide identification by scoring MS/MS spectra against peptides derived from a protein sequence database.
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Protein FDR #146

Closed qzhang503 closed 1 year ago

qzhang503 commented 1 year ago

I am new to MS-GF+. An earlier post indicated that QValue may be used for FDR controls at the levels of PSM. Is there a similar way to compile the PSM outputs to attain 1% protein FDR? Many thanks.

alchemistmatt commented 1 year ago

MS-GF+ does not compute a protein level FDR, just PSM level (QValue) and peptide level (PepQValue).

qzhang503 commented 1 year ago

Thank you for the note.

On Wed, Mar 29, 2023 at 2:06 PM Matthew Monroe @.***> wrote:

MS-GF+ does not compute a protein level FDR, just PSM level (QValue) and peptide level (PepQValue).

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