Closed cpfeifer58 closed 1 year ago
Hi @cpfeifer58!
Regarding the minimum length of the sequences: there is currently no minimum length, but I take your question as an incentive to include an additional sanity check on the sequence lengths. Thank you!
It seems your library file is not correctly set up. The first column should contain a unique gRNA identifier, and the second column should contain the actual gRNA sequence. Could you please try the file below and let me know if it works?
Worked like a charm! Thanks!
Hello!
When ReCo accesses a library for paired sample type, I encounter an error where all of my samples are considered invalid. Below is the error presented:
I'm including my library file as well in case it is formatted incorrectly, however is there a minimum length for sequences? In generating the library file I kept only the uniquely identifying portions of the gRNA.
unirecoLib1.csv
Please let me know if I can include any more information!