Closed hq66 closed 1 year ago
Hello,
The script 'inspector.py' only evaluates the assembly and identifies assembly errors. The 'valid_contig.fa' includes original uncorrected contigs. Error correction is done by running additional step of "inspector-correct.py -i inspector_out/ --datatype pacbio-hifi ". You will find corrected assembly in a file named 'corrected_contig.fa'.
Best, Maggi
Hello,
The script 'inspector.py' only evaluates the assembly and identifies assembly errors. The 'valid_contig.fa' includes original uncorrected contigs. Error correction is done by running additional step of "inspector-correct.py -i inspector_out/ --datatype pacbio-hifi ". You will find corrected assembly in a file named 'corrected_contig.fa'.
Best, Maggi
Thanks for your reply!
Hi~ I used the command "inspector.py -c ctg.fa -r ccs1.fq ccs2.fq -o inspector_out/ --datatype hifi" to evaluate the ctg-level draft assembly. But I found the output "valid_contig.fa" was exactly the same as the input "ctg.fa". I careflly compared the size of contigs and the base sequence in "small_scale_error.bed", and I'm sure the "valid_contig.fa" makes no difference. The "summary_statistics" did output errors, but I wonder why they were not fixed in "valid_contig.fa".